Isolation and bacterial expression of a sesquiterpene...

Details Isolation and bacterial expression of a sesquiterpene... Isolation and bacterial expression of a sesquiterpene...

2005-01-25

2005-01-25

Hutson, Richard (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

C435S320100, C435S419000, C435S252300, C435S252100, C530S350000, C536S023100, C536S023200

Reexamination Certificate

active

06846659

ABSTRACT:
A cDNA encoding (E)-β-farnesene synthase from peppermint (Mentha piperita) has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Accordingly, an isolated DNA sequence (SEQ ID NO:1) is provided which codes for the expression of (E)-β-farnesene synthase (SEQ ID NO:2), from peppermint (Mentha piperita). In other aspects, replicable recombinant cloning vehicles are provided which code for (E)-β-farnesene synthase, or for a base sequence sufficiently complementary to at least a portion of (E)-β-farnesene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding (E)-β-farnesene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant (E)-β-famesene synthase that may be used to facilitate its production, isolation and purification in significant amounts. Recombinant (E)-β-farnesene synthase may be used to obtain expression or enhanced expression of (E)-β-famesene synthase in plants in order to enhance the production of (E)-β-farnesene, or may be otherwise employed for the regulation or expression of (E)-β-farnesene synthase, or the production of its product.

REFERENCES:
Salin, F. et al.J. Plant Phys., 146:203-209 (1995).
Gershenzon, J. et al.,Anal. Biochem, 200:130-138 (1992).
Logemann, J. et al.,Anal. Biochem, 163:16-20 (1997).

Affiliated with

Also associated with

Rating

Isolation and bacterial expression of a sesquiterpene... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Isolation and bacterial expression of a sesquiterpene..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Isolation and bacterial expression of a sesquiterpene... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3416502