Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2006-05-15
2008-10-14
Sullivan, Daniel M (Department: 1636)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S069300, C435S069400, C435S069700, C435S070100, C435S070200, C435S070210, C435S070300, C435S071100
Reexamination Certificate
active
07435553
ABSTRACT:
A method of detecting and isolating cells that produce any secreted protein of interest (POI), comprising: a) constructing a cell line transiently or stably expressing a cell surface capture molecule, which binds the POI, by transfecting the cell line with a nucleic acid that encodes such cell surface capture molecule; b) transfecting said cell simultaneously or subsequently with a second nucleic acid that encodes a POI wherein such POI is secreted; c) detecting the surface-displayed POI by contacting the cells with a detection molecule, which binds the POI; and d) isolating cells based on the detection molecule.
REFERENCES:
patent: WO 94/09117 (1997-04-01), None
patent: WO 97/29131 (1997-08-01), None
patent: WO 99/58977 (1999-11-01), None
patent: 199 00 635 (2000-07-01), None
Manz, R., et al., (1995) Proc. Natl. Acad. Sci. USA 99:1921-1925.
Dangl and Herzenberg (1982) J. Immunol. Methods 52:1.
Gray, et al., (1995) J. Immunol. Methods 182:155.
Martel et al., (1988) J. Immunol. 141:1624.
Meng, et al., (2000) Gene 242:201.
Pallavacini, et al., (1989) J. Immunol. Methods 117:99.
Parks, et al., (1979) PNAS 76:1962.
Chen Gang
Fandl James P
Stahl Neil
Yancopoulos George D.
Gregg, Esq. Valeta
Regeneron Pharmaceuticals Inc.
Smeland, Esq. Tor E.
Sullivan Daniel M
Yang Ying-Zi
LandOfFree
Isolating cells expressing secreted proteins does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Isolating cells expressing secreted proteins, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Isolating cells expressing secreted proteins will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4012227