Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2005-07-19
2005-07-19
Guzo, David (Department: 1636)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S069300, C435S069400, C435S069700, C435S070100, C435S070200, C435S070210, C435S070300, C435S071100
Reexamination Certificate
active
06919183
ABSTRACT:
A method for identifying and isolating cells which produce secreted proteins. This method is based upon a specific characteristic or the expression level of the secreted protein by transiently capturing the secreted protein on the surface of an individual cell, allowing selection of rare cell clones from a heterogeneous population. Also provided is the use of this method to generate cells which produce a desired level of secreted protein or secreted protein of a particular characteristic(s), and organisms which possess such cells. In particular, the method allows rapid isolation of high expression recombinant antibody-producing cell lines, or may be applied directly to rapid isolation of specific hybridomas, or to the isolation of antibody-producing transgenic animals. This method is applicable for any cell which secretes protein.
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Chen Gang
Fandl James P.
Stahl Neil
Yancopoulos George D.
Gregg, Esq. Valeta
Guzo David
Regeneron Pharmaceuticals Inc.
Sullivan Daniel M.
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