Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Albumin
Patent
1998-07-31
1999-11-16
Naff, David M.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Albumin
426580, 426583, 426657, 530394, 530416, C07K 118, C07K 14435, A23J 120
Patent
active
059860634
ABSTRACT:
A method is provided for isolating the proteins, .beta.-lactoglobulin and .alpha.-lactalbumin, from whey with a single cation exchanger, and using different pH values for eluting the proteins as separate fractions without using salt elution. A whey protein solution is adjusted to a pH of less than about 4.5. The solution is contacted with a cation exchanger to obtain a bound fraction containing .alpha.-lactalbumin and .beta.-lactoglobulin. The bound fraction is adjusted to a pH of about 4.0 to 6.0 and a .beta.-lactoglobulin fraction is eluted at this pH in the absence of sodium chloride. The pH of an remaining bound fraction is adjusted to about 6.5 or greater and an .alpha.-lactalbumin fraction is eluted. The method is advantageously conducted at elevated temperatures ranging from 35.degree. C. to 50.degree. C. The ion exchanger may be cross-linked polymeric beads made of cellulose, agarose or dextran, or a microporous polymeric membrane made of regenerated cellulose, polysulfone or cellulose acetate, and may contain charged immobilized molecules such as carboxymethyl or sulfopropyl moieties.
REFERENCES:
patent: 5516675 (1996-05-01), Uchida et al.
Naff David M.
Wisconsin Alumni Research Foundation
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