Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Primate cell – per se
Patent
1995-05-10
1998-12-15
Witz, Jean C.
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Primate cell, per se
435366, 435363, 435325, 435384, 435389, 435387, C12N 506
Patent
active
058495851
ABSTRACT:
A method for enhancing the survival and/or proliferation of Schwann cells (especially human Schwann cells) in cell culture is disclosed which involves culturing the cells in serum free culture medium comprising gas6 and other mitogenic agents, such as heregulin and forskolin. The culturing step is generally preceded by a pre-incubation period wherein nerve tissue comprising the Schwann cells is cultured under appropriate conditions and for a period of time such that demyelination occurs. The isolated Schwann cells can be used as cellular prostheses to treat patients with nervous system injuries. The invention also provides a cell culture medium for culturing Schwann cells.
REFERENCES:
patent: 4443546 (1984-04-01), Stemerman et al.
patent: 4662884 (1987-05-01), Stensaas et al.
patent: 5030225 (1991-07-01), Aebischer et al.
patent: 5082670 (1992-01-01), Gage et al.
patent: 5147790 (1992-09-01), Wilson
patent: 5326699 (1994-07-01), Torishima et al.
patent: 5342777 (1994-08-01), Cole et al.
Sigma Cell Culture Catalogue, pp. 46, 54, 63, 68, and 70, 1994.
Rutkowski, J.L. et al., "Ann. Neurol.," vol. 31, pp. 580-586, 1992.
Zhang, B.T. et al., "J. of Neuroscience Res.," vol. 41, pp. 648-654, 1995.
Tong, Z.B., "Yokohama Igaku," vol. 46(2), pp. 111-117. English Translation and original article, 1995.
Stewart, H.J.S. et al., "J. of Neuroscience Res.," vol. 30(2), pp. 346-352, Oct. 1991.
Needham, L.K. et al., "The J. of Neuroscience," vol. 7(1), pp. 1-9, 1987.
Eccleston, P.A. et al., "Developmental Biology," vol. 124(2), pp. 409-417, 1987.
Ohashi, K. et al., "The J. of Biological Chem.," vol. 270(39), pp. 22681-22684, Sep. 1995.
Sigma Cell Culture Reagent Catalogue/Price List pp. 56 and 65 (1992).
Bhattacharyya et al., "Axons Arrest the Migration of Schwann Cell Precursors" Development 120:1411-1420 (1994).
Li et al., "Identification of Gas6 as a Growth Factor for Human Schwann Cells" The Journal of Neuroscience 16(6):2012-2019 (Mar. 15, 1996).
Morrissey et al., "Axon-induced mitogenesis of human Schwann cells involves heregulin and p185erbB2" Proc. Natl. Acad. Sci. USA 92:1431-1435 (Feb. 1995).
Roberts et al., "A novel epithelial cell from neonatal rat lung:isolation and differentiated phenotype" Amer. J. Physiol.:Lung Cell. & Molec. Physiol 259(3):L415-L425 (1990).
Son et al., "Schwann Cell Processes Guide Regeneration of Pheripheral Channels Axons" Neron 14:125-132 (Jan. 1995).
Xu et al., "Axonal Regeneration Into Schwann Cell-seeded Guidance Channels Grafted Into Transected Adult Rat Spinal Cord" J. Comp. Neuro. 351:145-160 (1995).
Bellosta et al., "The Receptor Tyrosine Kinase ARK Mediates Cell Aggregation by Homophilic Binding" Molecular & Cellular Biology 15(2):614-625 (Feb. 1995).
Brockes, "Assay and isolation of glial growth factor from the bovine pituitary" Methods in Enzymology 147:217-225 (1987), Peptides Growth Factors, part B ed. D. Bones et al. AP, Inc.
Brockes et al., "Glial growth factor-like activity in Schwann Cell tumors" Annals of Neurology 20:317-322 (1986).
Brockes et al., "Purification and preliminary characterization of a glial growth factor from the bovine pituitary" Journal of Biological Chemistry 255(18):8374-8377, Sep. (1980).
Brockes et al., "Studies on cultured rat schwann cells. I. Establishment of purified populations from cultures of peripheral nerve" Brain Research 165:105-118 (1979).
Brummendorf et al., "Axonal Glycoproteins with Immunoglobulin- and Fibronectin Type III-Related Domains in Vertebrates: Structural Features, Binding Activities and Signal Transduction" Journal of Neurochemistry 61(4):1207-1219 (1993).
Bunge, "Expanding roles for the Schwann cell: ensheatment, myelination, trophism and regeneration" Current Opinion in Neurobiology 3:805-809 (1993).
Collier et al., "Schwann Cells as a Source of Neurotophic Activity for Dopamine Neurons" Exper. Neurol. 124:129-133 (1993).
Colombo et al., "Localization of Growth arrest-specific genes on mouse Chromosomes 1, 7, 8, 11, 13, and 16" Mammalian Genome 2:130-134 (1992).
Dahlback et al., "High molecular weight complex in human plasma between vitamin K-dependent protein S and complement component C4b-binding protein" Proc. Natl. Acad. Sci. USA 78(4):2512-2516 (Apr. 1981).
Dai et al., "Molecular Cloning of a Novel Receptor Tyrosine Kinase, tif, Highly Expressed in Human Ovary and Testis" Oncogene 9:975-979 (1994).
Esmon, "The Protein C Anticogulatant Pathway" Arteriosclerosis and Thrombosis 12(2):135-145 (Feb. 1992).
Ferrero et al., "Expression of Growth Arrest Specific Gene (gas-6) During Liver Regeneration: Molecular Mechanisms and Signalling Pathways" Journal of Cellular Physiology 158:263-269 (1994).
Fujimoto, "brt, A Mouse Gene Encoding a Novel Receptor-type Protein-Tyrosine Kinase, is Preferentially Expressed in the Brain" Oncogene 9:693-698 (1994).
Gasic et al., "Coagulation factors X, Xa, and protein S as potent mitogens of cultured aortic smooth muscle cells" Proc. Natl. Acad. Sci. USA 89:2317-2320 (Mar. 1992).
Graham et al., "Cloning and mRNA Expression Analysis of a Novel Human Protooncogene, c-mer.sup.1 " Cell Growth & Differentiation 5:647-657 (Jun. 1994).
Griffin et al., "Reevaluation of Total, Free and Bound Protein S and C4b-Binding Protein Levels in Plasma Anticogulated With Citrate or Hirudin" Blood 79(12) :3203-3211 (Jun. 1992).
Guenard et al., "Syngenic Schwann Cells Derived from Adult Nerves Seeded in Semipermeable Guidance Channels Enhance Peripheral Nerve Regeneration" Journal of Neuroscience 12(9):3310-3320 (1992).
Hammond et al., "The cDNA-deduced primary structure of human sex hormone-binding globulin and location of its steroid-binding domain" FEBS Letters 215(1):100-104 (May 1987).
Haynes et al., "Diploid and hyperdiploid rat Schwann cell strains displaying negative autoregulation of growth in vitro and myelin sheath-formulation in vivo" Journal of Neuroscience Methods 52:119-127 (1994).
Janssen et al., "A novel putative tyrosine kinase receptor with oncogenic potential" Oncogene 6:2113-2120 (1991).
Joseph et al., "Sex hormone-binding globulin, androgen-binding protein, and vitamin K-dependent protein S are homologous to laminin A, merosin, and Drosophila crumbs protein" FASEB J. 6:2477-2481 (1992).
Lai et al., "Structure, expression, and activity of Tyro 3, a neural adhesion-related receptor tyrosine kinase" Oncogene 9:2567-2578 (1994).
Levi et al., "The functional characteristics of Schwann Cells culture from human peripheral nerve after transplantation into a gap within the rat sciatic nerve" J. Neuroscience 14(3):1309-1319 Mar. (1994).
Levi et al., "The influence of Heregulins on Human Schwann Cell Proliferation" J. Neuroscience 15(2):1329-1340 (Feb. 1995).
Li et al, "Schwann Cells Induce Sprouting in Motor and Sensory Axons in the Adult Rat Spinal Cord" Neuroscience 14(7):4050-4063 (Jul. 1994).
Manfioletti et al., "The protein encoded by a growth arrest-specific gene (gas6) is a new member of the vitamin K-dependent proteins related to protein S, a negative coregulator in the blood coagulation cascade" Molecular & Cellular Biology 13(8):4976-4985 (Aug. 1993).
Mark et al., "rse, a Novel Receptor-type Tyrosine Kinase with Homology to Axl/Ufo, Is Expressed at High Levels in the Brain" Journal of Biological Chemistry 269(14):10720-10728 (Apr. 8, 1994).
Messing et al., "Hypomeylinating Peripheral Neurophathies and Schwannomas in Transgenic Mice Expressing SV40 T-Antigen" Journal of Neuroscience 14(6):3533-3539 (Jun. 1994).
Morgan et al., "Negative regulation of the P.sub.o gene Schwann cells: suppression of .sub.o mRNA and protei induction in cultured Schwann cells by FGF2 and TGF.beta.1, TGF.beta.2, and TGF.beta.3" Development 120:1399-1409 (1994).
Morrissey et al., "Isolation and Fuctional characterization of Schwann Cells Derived from Adult Peripheal Nerve" Journal of Neuroscience 11(8):2433-2442 (Aug. 1991).
O'Bryan et al., "axl, a transforming gene isolated from primary human myeloid leukemia cells, encodes a novel receptor tyrosine kinase" Molecular & Cellular Biology 11(10):5016-5031 (Oct. 1991).
Ohashi et al., "Cloning of the cDNA for a Novel Receptor Tyrosine Kinase,
Chen Jian
Li Rong-hao
Mather Jennie P.
Genetech, Inc.
Lee Wendy M.
Witz Jean C.
LandOfFree
Isolating and culturing Schwann cells does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Isolating and culturing Schwann cells, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Isolating and culturing Schwann cells will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1457134