Isolated xylanase gene with mutations and site-specific...

Details Isolated xylanase gene with mutations and site-specific... Isolated xylanase gene with mutations and site-specific...

2007-09-07

2009-11-10

Fronda, Christian L (Department: 1652)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C435S183000, C435S201000, C435S252300, C435S320100, C536S023200

Reexamination Certificate

active

07615362

ABSTRACT:
A mutated xylanase gene with a broad pH range of reaction includes a forty-first amino acid or a twenty-first amino acid generated from transforming asparagine to aspartic acid so as to form the mutated xylanase gene. A site-specific mutagenesis method includes the step of: mutating the forty-first amino acid or the twenty-first amino acid of the xylanase gene by transforming asparagine to aspartic acid so as to form the mutated xylanase gene.

REFERENCES:
Chica et al. Curr Opin Biotechnol. Aug. 2005;16(4):378-84.
Sen et al. Appl Biochem Biotechnol. Dec. 2007;143(3):212-23.
Accession Q3KT08, published Nov. 8, 2005.
Khandeparker et al. J Ind Microbiol Biotechnol. Jul. 2008;35(7):635-44. Epub Mar 26, 2008.

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