Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of...
Patent
1997-02-20
2000-01-25
Marx, Irene
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
424 931, 424 937, C12P 506
Patent
active
060177578
ABSTRACT:
The present invention provides a homogenous population of parasite cells, wherein the cells are not mosquito cells, capable of prolonged culture in vitro. Fractions from the cells, both cellular and non-cellular are also provided. Further provided is a method of treating or preventing parasite involvement with an animal comprising administering immunogenic amounts of the parasite cells of the invention or fractions derived from the cells to the animal, thereby treating or preventing the parasite involvement with the animal. Further provided is a method of detecting the presence of a parasite in an animal, comprising contacting either the parasite cells or cell fractions of the invention or antibodies of the invention with either an antibody containing sample or an antigen containing sample from the animal and detecting the presence of binding of either the antibodies in the sample with the cells or fractions of the invention or the binding of the antigens in the sample with the antibodies of the invention, the presence of binding indicating the presence of a parasite in the animal. In addition, the invention provides a method of culturing parasite cell populations in vitro comprising culturing a parasite in parasite culture medium under conditions which allow for decomposition and/or degradation of the cuticle layer of the parasite such that cellular buds are produced; disrupting the culture to cause the cellular buds to shear from the cuticle layer; and culturing the parasite cellular buds in cell culture medium. Finally provided is a population of differentiated nematode cells capable of prolonged culture in vitro.
REFERENCES:
patent: 4447537 (1984-05-01), Yunker et al.
patent: 5094954 (1992-03-01), Previc
Gordon et al., Can. J. Zool. (1990), 68(3), 511-16.
Franke et al., Science, (Jul. 8, 1983) 221 (4606) 161-3.
Hirumi et al., Phytopathology 59 (11). 1969.
Sanders et al., Nematologica 19 (4). 1973 567-568.
Rickard et al., In "In Vitro Methods for Parasite Cultivation", Academic Press, Taylor, A. E. R. and J. R. Baker (Ed.), 1987. 407-451.
Lewis et al., Methods Cell Biol. (1995), Volume Date 1995, 48, 3-29.
Gamble H. and Mansfield L., "Characterization of excretory-secretory products from larval stages of Haemonchus contortus cultured in vitro", Veterinary Parasitology 62:291-305, 1996.
Gamble et al., "Developmentally regulated zinc metalloproteinases from third-and fourth-stage larve of the ovine nematode Haemonchus controtus," J. Parasitol. 82(2):197-202, 1996.
Manousis, T. and Ellar, D. J. "Establishment of two cells lines from the nematode Meloidogyne incognita (Tylenchida; Meloidogynidae)" In Vitro Cell Dev. Biol. 26:1105-1114, Nov. 1990.
Kurtti, T. J. and Munderloh, U. G. "Mosquito cell culture", Advances in Cell Culture, 3:259-302, 1984.
Hobbs et al. "Culture of Cells from Juvenile Worms of Schistosoma Mansoni", J. Parasitol. 79(6):913-921, 1993.
Jenkins et al. "Fasciola hepatica in vitro: increased susceptibility to fasciolicides in a defined serum-free medium", Parasitology 95:165-171, 1987.
Howell, M.J. An Approach to the Production of Helminth Antigens in vitro: The Formation of Hybrid Cells Between Fasciola Hepatica and a Rat Fibroblast Cell Line, Int. J. Parasitol. 11(3):235-242, 1981.
Marx Irene
Mississippi State University
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