Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease
Reexamination Certificate
1998-08-24
2001-06-12
Saidha, Tekchand (Department: 1652)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Transferase other than ribonuclease
C435S252300, C435S320100, C435S468000, C435S069100, C435S070100, C536S023200, C536S023600, C800S278000, C800S285000, C800S298000, C530S350000
Reexamination Certificate
active
06245541
ABSTRACT:
BACKGROUND OF THE INVENTION
1. Field of the Invention
This invention relates to ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit (LS)
&egr;
N-methyltrnnsferase (protein methylase III or Rubisco LSMT). This enzyme catalyzes methylation of the &egr;-amine of lysine-14 in the large subunit of Rubisco. Many plant species contain methylated Lys-14 in the LS of Rubisco but some do not (i.e., a des(methyl) lysyl residue in the LS). In addition, the present invention relates to a gene and full-length cDNA clones for Rubisco LSMT. The present invention further relates to transgenic plants and methods of producing same which have the Rubisco LSMT gene inserted into the DNA. This invention also relates to a four amino acid insert (WVQQ) which inactivates the enzymatic activity of Rubisco LSMT and thereby accounts for the subsequent absence of trimethyllysine-14 in the LS of Rubisco.
2. Description of the Related Art
Protein methylation is a widespread and common post-tanslational modification catalyzed by several different protein methyltransferases (Paik et al., “Protein methylation,” in Freedman et al. (eds),
The Enzymology of Posttranslational Modifications of Proteins
, vol. 2, pp. 187-228, Academic Press, London (1985)). Proteins which contain trimethyllysyl residues include cytochrome c (Cessay et al., “The relationship between the trimethylation of lysine 77 and cytochrome c metabolism in
Saccharomyces cerevisiae,” Int. J. Biochem.
26(5):721-734 (1994); Cessay et al., “Further investigations regarding the role of trimethyllysine for cytochrome c uptake into mitochondria,”
Int. J. Biochem.
23(7,8): 761-768 (1991); DiMaria et al., “Cytochrome c specific methylase from wheat germ,”
Biochemistry
21:1036-1044 (1982); Farooqui et al., “Effect of Methylation on the Stability of Cytochrome c of
Saccharomyces cerevisiae
in vivo,”
J. Biol. Chem.
256(10):5041-5045 (1981); and Farooqui et al., “In vivo studies on yeast cytochrome c methylation in relation to protein synthesis,”
J. Biol. Chem.
255(10):4468-4473 (1980)), calmodulin (Han et al., “Isolation and kinetic characterization of the calmodulin mnethyltransferase from sheep brain,”
Biochemistry
32:13974-13980 (1993); and Rowe et al., “Calmodulin N-methyltransferase,”
J. Biol. Chem.
261(15):7060-7069 (1986)), histone-H1 (Sarnow et al., “A histone H4-specific methyltransferase properties, specificity and effects on nucleosomal histones,”
Biochim. Biophys. Acta
655:349-358 (1981); and Tuck et al., “Two histone H1-specific protein-lysine N-methyltransferases from
Euglena gracilis, ” J. Biol. Chem.
260(11):7114-7121 (1985)), and ribosomal proteins (Chang et al., “Purification and properties of a ribosomal protein methylase from
Escherichia coli
Q13,
” Biochemisry
14(22):4994-4998 (1975); Lobet et al., “Partial purification and characterization of the specific protein-lysine N-methyltransferase of YL32, a yeast ribosomal protein,”
Biochim. Biophy. Acta
997:224-231 (1989)). However, the biological function of post-translational protein methylation in all but a few systems remains obscure. Trimethyllysine can serve as a metabolic precursor to carnitine (Paik et al., “Carnitine biosynthesis via protein methylation,”
TIBS
2: 159-162 (1977)), while carboxyl methylation of bacterial membrane proteins plays a major role in chemotaxis (Clarke, “Protein carboxyl methyltransferases: Two distinct classes of enzymes,”
Ann. Rev. Biochem.
54: 479-506 (1985)). Evidence suggests that methylation of Lys-115 in calmodulin affects certain activities including in vitro NAD kinase activation (Roberts et al., “Trimethyllysine and protein function,”
J. Biol. Chem.
261(4):1491-1494 (1986)), and in vivo susceptibility to ubiquitination (Gregori et al., “Bacterially synthesized vertebrate calmodulin is a specific substrate for ubiquitination,”
J. Biol. Chem.
262(6):2562-2567 (1987); and Gregori et al., “Specific recognition of calmodulin from
Dictyostelium discoideum
by the ATP ubiquitin-dependent degradative pathway,”
J. Biol. Chem.
260(9):5232-5235 (1985); but see also Ziegenhagen et al., “Multiple ubiquitination of calmodulin results in one polyubiquitin chain linked to calmodulin,”
FEBS. Lett.
271(1,2):71-75 (1990); and Ziegenhagen et al., “Plant and fungus calmodulins are polyubiquitinated at a single site in a Ca
2+
-dependent manner,”
FEBS Lett.
273(1,2):253-256 (1990)). Conflicting reports (Farooqui et al., “Effect of Methylation on the Stability of Cytochrome c of
Saccharomyces cerevisiae
in vivo,”
J. Biol. Chem.
256(10):5041-5045 (1981); Frost et al., “Cytochrome c methylation,”
Protein methylation
, Ch. 4, pp. 59-76 (1990); and Frost et al., “Effect of enzymatic methylation of cytochrome c on its function and synthesis,”
Int. J. Biochem.
22(10): 1069-1074 (1990); versus Cessay et al., “The relationship between the trimethylation of lysine 77 and cytochrome c metabolism in
Saccharomyces cerevisiae,” Int. J. Biochem.
26(5):721-734 (1994); Cessay et al., “Further investigations regarding the role of trimethyllysine for cytochrome c uptake into mitochondria,”
Int. J. Biochem.
23(7,8):761-768 (1991)) also implicate methylation of Lys-77 in cytochrome c as having a role in protein stability, heme incorporation, and mitochondrial transport. A major limitation to elucidating the biological role of lysine methylation in eukaryotes has been the absence of a protein methylase III gene. Hence, molecular studies of the physiological and biochemical function performed by methylation of protein bound lysyl residues have been restricted to site-directed mutational analysis of the methylation site in the target protein (Ceesay et al., “The relationship between the trimethylation of lysine 77 and cytochrome c metabolism in
Saccharomyces cerevisiae,” Int. J. Biochem.
26(5):721-734 (1994); Cessay et al., “Further investigations regarding the role of trimethyllysine for cytochrome c uptake into mitochondria,”
Int. J. Biochem.
23(7,8):761-768 (1991); and Roberts et al., “Expression of a calmodulin methylation mutant affects the growth and development of transgenic tobacco plants,”
Proc. Nat. Acad. Sci. USA
89:8394-8398 (1992)). These studies have been inconclusive as to the exact biological role of methylation of the &egr;-amine of protein bound lysyl residues.
Ribulose-1,5-bisphosphate carboxylase-oxygenase (Rubisco) catalyzes the reduction of atmospheric CO
2
during photosynthesis. A great deal is known about the quaternary structure, catalytic mechanism, active site residues, in vivo regulatory mechanisms, and gene expression for this abundant enzyme, see, for example, Andrews et al., “Rubisco: Structure, Mechanisms, and Prospects for Improvement,” in Hatch et al. (eds),
The Biochemistry of Plants
, vol, 10, pp. 131-218. Academic Press, York (1987); Dean et al., “Structure, evolution, and regulation of rbcS genes in higher plants,”
Annu. Rev. Plant. Physiol. Plant Mol. Biol.
40: 415-439 (1989); and Mullet, “Chloroplast development and gene expression,”
Annu. Rev. Plant. Physiol. Plant Mol. Biol.
39: 475-502 (1988). Higher plant Rubisco is a hexadecameric protein composed of eight chloroplast-encoded large subunits (referred to herein as “LS”) and eight nuclear-encoded small subunits (referred to herein as “SS”). Synthesis of the LS is accompanied by post-translational processing of the N-terminal domain (Houtz et al., “Post-translational modifications in the large subunit of ribulose bisphosphate carboxylase/oxygenase,”
Proc. Natl. Acad. Sci. USA
86:1855-1859 (1989); and Mulligan et al., “Reaction-intermediate analogue binding by ribulose bisphosphate carboxylase/oxygenase causes specific changes in proteolytic sensitivity: The amino-terminal residue of the large subunit is acetylated proline,”
Proc. Natl. Acad. Sci. USA
85:1513-1517 (1988)). The N-terminal Met-1 and Ser-2 are removed and Pro-3 acetylated. Additionally, the LS of Rubisco from tobacco, muskmelon, pea, and several other species is post-translationally modified by trimethylation of the &egr;-amine of Lys-14 (Houtz e
Burns Doane Swecker & Mathis L.L.P.
Saidha Tekchand
University of Kentucky Research Foundation
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