Isolated recombinant uricase

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase

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C12N 906

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active

057285624

ABSTRACT:
This invention relates to an isolated uricase which is stable in an aqueous solution at a temperature up to 60.degree. C. and at a pH of 8.0 for 10 minutes.

REFERENCES:
patent: 3475276 (1969-10-01), Kano
patent: 4273874 (1981-06-01), Nakanishi et al.
patent: 4882280 (1989-11-01), Takashio et al.
patent: 4987076 (1991-01-01), Takashio et al.
L.G. Kral et al, "Cloning a cDNA for Drosphila melanogaster urate oxidase", Gene, vol. 45, 131-173, (1986).
C.C. Lee et al, "Generation of cDNA Probes Directed by Amino Acid Sequence: Cloning of Urate Oxidase", Science, vol. 239, 1288-1291 (1988).
T. Nguyen et al, "Primary structure of the soybean nodulin-35 gene encoding uricase II localized in the peroxisomes of uninfected cells of nodules", Proc. Natl. Acad. Sc., vol. 82, 5040-5044 (1985).
M. Ito et al, "T. Nucleotide sequence of cDNA and predicted amino acid sequence of rat liver uricase", Eur. J. Biochem., vol. 173, 459-463 (1988).
Suggs et al "Use of synthetic oligonucleotides as hybridization Probes . . ", PNAS, vol. 78, No. 11, pp. 6613-6617, (1981).
Young, et al, "Efficient isolation of genes by using antibody probes", PNAS, vol. 80, Mar. 1983, pp. 1194-1198.
Nippon Nogeikagaku Kaishi, vol. 63, No. 3 p. 163 (1989), The Agricultural Society of Japan, Mar. 15, 1989 and English language translation thereof.

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