Isolated protein having hydroxynitrile lyase activity

Details Isolated protein having hydroxynitrile lyase activity Isolated protein having hydroxynitrile lyase activity

2007-04-10

2007-04-10

Saidha, Tekchand (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Lyase

C435S041000, C435S183000, C435S320100, C530S350000, C536S023200, C558S351000

Reexamination Certificate

active

10940954

ABSTRACT:
New genes containing a DNA sequence coding for hydroxynitrile lyase, which genes can be prepared via a primer combination based on the DNA sequence of the 5′-region of the mdl genes from thePrunus serotinaand fromPrunus amygdalusand/or a primer 2 based on the 3′-region of the DNA sequences of one of the hydroxynitrile lyase isoenzymes fromPrunus serotinaor fromPrunus amygdalus, subsequent amplification with a DNA polymerase using a DNA from organisms, containing genes coding for hydroxynitrile lyase, as templates and cloning, and also recombinant proteins which can be prepared in suitable host cells by heterologous expression of the DNA sequence of said HNL genes, and proteins and fusion proteins derived therefrom and use of said proteins for preparing (R)- or (S)-cyanohydrins.

REFERENCES:
patent: 6046042 (2000-04-01), Hasslacher et al.
Cheng et al. Accession S32156. Nov. 22, 1993.
M. Suelves, et al., “Molecular cloning of the cDNA coding for the (R)-(+)-mandelonitrile lyase ofPrunus amygdalus: temporal and spatial expression patterns in flowers and mature seeds”,Planta, vol. 206, pp. 388-393 (1998).
Z. Hu et al., “Molecular Analysis of (R)-(+)-Mandelonitrile Lyase Microheterogeneity in Black Cherry”,Plant Physiology, vol. 119, pp. 1535-1546 (Apr. 1999).
Z. Hu et al., Accession U51562, Apr. 11, 1996.
ExPASy Home Page, “NiceZyme View of ENZYME: 4.1.2.10”, Mandelonitrile lyase.
T. K. Atwood, et al., “Which craft is best in bioinformatics?”,Computers and Chemistry, vol. 25, pp. 329-339 (2001).
C. P. Ponting, “Issues in predicting protein function from sequence”,Briefings in Bioinformatics, vol. 2, No. 1, pp. 19-29 (Mar. 2001).

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