Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Reexamination Certificate
2011-05-17
2011-05-17
Zheng, Li (Department: 1638)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
C800S279000
Reexamination Certificate
active
07943339
ABSTRACT:
Disclosed are nucleic acids from the Citrus Leprosis Virus (CiLV) genome, associated with Citrus Leprosis (CiL) disease. Cloned CiLV nucleic acids can be used as probes or primers in assays for detecting CiLV in biological samples, particularly leaves, roots and other tissues of plants, such as plants fromCitrusandPoncirus. The nucleic acid molecules can be introduced in cloning vectors and cloning the recombinant nucleic acid molecules in cells, such as prokaryotes, and eukaryotes. The cloned CiLV nucleic acids are expressed in cells to provide immunogenic proteins which can be used to raise antibodies against CiLV, which can then be used to detect CiLV virus in biological samples. Also disclosed are SEQ ID Nos. 5 and 8, in whole or part, transgenic plants, such as monocots and dicots, containing CiLV nucleic acids, in any combination, so that expression increases resistance to CiL disease.
REFERENCES:
Locali et al. 2003, Plant Disease 87:1317-1321.
Locali et al 2003 Plant Disease 87:1317-1321.
Locali et al. Nov. 2003, Plant Disease 87:1317-1321.
Fourgoux-Nicol et al 1999, Plant Molecular Biology 40 :857-872.
Eliane Cristina Locali et al., “Development of a Molecular Tool for the Diagnosis of Leprosis, a Major Threat to Citrus Production in the Americas”, Plant Disease, vol. 87, No. 11, Nov. 2003, pp. 1317-1321.
E.W. Kitajima et al., “Brevipalpus- transmitted plant virus and virus like diseases: cytopathology and some recent cases”, Experimental and Applied Acarology 30: 135-160, 2003.
J.C.V. Rodrigues et al., “Citrus leprosis virus vectored byBrevipalpus phoenicis(Acari: Tenuipalpidae) on citrus in Brazil”, Experimental and Applied Acarology 30:161-179, 2003.
E.C. Locali-Fabris et al., “Complete nucleotide sequence, genomic organization and phylogenetic analysis of Citrus leprosis virus cytoplasmic type”, Journal of General Virology (2006) 87, 2721-2729.
Renata C. Pascon et al., “The complete nucleotide sequence and genomic organization of Citrus Leprosis associated Virus, Cytoplasmatic type (CiLV-C)”, Virus Gene (2006) 32:289-298.
Pascon Renata Castiglioni
Silva Ana Claudia Rasera
Alellyx S.A.
Bent Stephen A.
Foley & Lardner LLP
Zheng Li
LandOfFree
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