Isolated nucleic acid molecules encoding the p27 KIP-1 protein

Details Isolated nucleic acid molecules encoding the p27 KIP-1 protein Isolated nucleic acid molecules encoding the p27 KIP-1 protein

1994-07-15

1997-11-18

Allen, Marianne P.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4353201, 4352523, 4352401, 536 235, 935 11, C12N 1512, C12N 510, C12N 121

Patent

active

056886659

ABSTRACT:
The subject invention provides an isolated protein having an apparent molecular weight of about 27 kD and capable of binding to and inhibiting the activation of a cyclin E-Cdk2 complex. The subject invention further provides a recombinant nucleic acid molecule which encodes the p27 protein of the subject invention, and related vectors and host vector systems. The subject invention further provides a method for producing the p27 protein of the subject invention using the host vector system. The subject invention further provides methods of determining whether an agent is capable of specifically inhibiting or enhancing the ability of p27 protein to inhibit the activation of cyclin E-Cdk2 complex. Finally, this subject invention provides different uses of the isolated protein, the recombinant nucleic acid molecule encoding the isolated protein and the agent capable of inhibiting or enchancing the ability of p27 protein to inhibit the activation of cyclin E-Cdk2 complex.

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Harper et al. (1993), "The p21 Cdk-Interacting Protein Cip1 is a Potent Inhibitor of G1 Cyclin-dependent Kinases," Cell vol. 75: 805-816.
Koff et al. (1992), "Formation and Activation of a Cyclin E-cdk2 Complex During the G.sub.1 Phase of the Human Cell Cycle," Science, vol. 257:1689-1694.
Polyak et al. (1994), "Cloning of p27.sup.kip1, a Cyclin-dependent Kinase Inhibitor and a Potential Mediator of Extracellular Antimitogenic Signals," Cell, vol. 78: 59-63.
Toyoshima et al. (1994) "p27, a Novel Inhibitor of G1 Cyclin-Cdk Protein Kinase Activity, is Related to p21," Cell, vol 78: 67-74.
Firpo et al. (1994), "Inactivation of a Cdk2 Inhibitor During Interleukin 2-induced Proliferation of Human T Lymphocytes," Molecular and Cellular Biology, vol. 14; 4889-4901.
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