Isolated genomic polynucleotide fragments from chromosome...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase

Reexamination Certificate

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C435S006120, C435S069100, C435S252300, C435S320100, C977S704000

Reexamination Certificate

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10457715

ABSTRACT:
The invention is directed to an isolated genomic polynucleotide fragment that encodes human soluble (cytosolic) aminopeptidase P, vectors and hosts containing the fragment and fragments hybridizing to noncoding regions as well as antisense oligonucleotides to these fragments. The invention is further directed to methods of using these fragments to obtain human soluble aminopeptidase P and to diagnose, treat, prevent and/or ameliorate a pathological disorder.

REFERENCES:
patent: 6399349 (2002-06-01), Ryan et al.
patent: 6812339 (2004-11-01), Venter et al.
patent: 2004/0265849 (2004-12-01), Cargill et al.
Printed alignment of the nucleotide sequences of SEQ ID No. 13,390 of US Patent No. 6,399,349 and SEQ ID No. 2 of this application.
Sprinkle, T.J., et al., 1998, “Assignemtn of the membrane-bound human aminopeptidase P gene (XPNPEP2) to chromosome Xq25”, Genomics, vol. 50, pp. 114-116.
Cottrell, G.S., et al., 2000, “Cloning, expression, and characterization of human cytosolic aminopeptidase P: a single Manganese(II)-dependent enzyme”, Biochemistry, vol. 39, pp. 15121-15128.
Sprinkle, T.J., et al., 2000, “Cloning, chromosomal sublocalization of the human soluble aminopeptidase P Gene (XPNPEP1) to 10q25.3 . . . ”, Archives of Biochemistry and Biophysics, vol. 387, No. 1, pp. 51-56.

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