Isolated fungal promoters and gene transcription terminators...

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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C435S006120, C435S069100, C435S320100

Reexamination Certificate

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07449569

ABSTRACT:
The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.

REFERENCES:
Roberts et al. Expression of theEscherichia colibeta-glucuronidase gene in industrial and phytopathogenic filamentous fungi. Curr. Genet. 15:177-180, 1989.
U.S. Appl. No. 10/442,017, Lasure et al.
Ebert et al. Proceedings of the National Academy of Sciences USA, 1987, 5745-5749, vol. 87.
Dai et al., “Identification of genes associated with morphology inAspergillus niger. . . ,” Applied Environmental Microbiology, 2004, 2474-2485, vol. 70.
Bennett and Lasure, eds., “More Gene Manipulations in Fungi,” 1991, 441-458, Academic Press Inc, San Diego, USA.
Jefferson et al., European Molecular Biology Organization Journal, 1987, 3901-3907, vol. 6.

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