Chemistry: molecular biology and microbiology – Vector – per se
Patent
1994-12-01
1997-03-18
Fleisher, Mindy
Chemistry: molecular biology and microbiology
Vector, per se
435243, 435325, 435367, 435358, 435352, 536 235, C12N 100, C12N 510, C12N 1511, C12N 1563
Patent
active
056122209
ABSTRACT:
Disclosed is a POU transcription factor, Sprm-1 and analogs thereof, DNA molecules encoding the Sprm-1 proteins, and chimeric constructs, vectors and host cells containing the DNA molecules. Also disclosed are methods to identify putative male contraceptive agents. One involves the steps of isolating a DNA regulatory region responsive to Sprm-1, stably transforming a heterologous cell line with a first chimeric DNA construct containing a constitutive promoter capable of functioning in the cell line operably linked to a Sprm-1 DNA and a second chimeric DNA construct containing the DNA regulatory region operably linked to a reporter gene. The thus-transformed cell line is cultural in media containing the compound so that the thus-cultured cell line can be assayed to determine the Sprm-1 inhibitory activity of the compound. The above method can be modified to evaluate the Sprm-1 inhibitory activity of DNA molecules of interest, by transiently transfecting the thus-transformed cell line with a third DNA construct containing a constitutive promoter capable of functioning in the cell line operably linked to the DNA molecule, and then culturing the thus-transfected cell line so that it can be assayed to determine the inhibitory activity of the DNA molecule. Antibodies specific to Sprm-1, and their use in diagnosing male germ cell Sprm-1-mediated diseases or conditions are further disclosed.
REFERENCES:
Verrijzer et al, The EMBO Journal, 1992, vol. 11(13): pp. 4993-5003.
Ringheim, Studies in Family Planning 24(2): 87-99 (1993).
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World Health Organization, "Challenges in reproductive health research," Biennial Report 1992-1993, pp. 72-76, 129-133.
World Health Organization, "Reproductive health: a key to a brighter future", Biennial Report 1990-1991, Special 20th anniversary issue, 93-96.
World Health Organization, "Annual TEchnical Report", Geneva, 1992, 59-68, 73-75
Andersen Bogi
Bardin C. Wayne
Pearse Richard V.
Rosenfeld Michael G.
Schlegel Peter N.
Fleisher Mindy
McKelvey Terry A.
The Population Council, Center for Biomedical Research
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