Isolated DNA encoding the NotI restriction endonuclease and rela

Details Isolated DNA encoding the NotI restriction endonuclease and rela Isolated DNA encoding the NotI restriction endonuclease and rela

1993-01-11

1994-12-06

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

4353201, 43525233, 435193, 536 232, C12N 922, C12N 1555, C12N 1570

Patent

active

053710061

ABSTRACT:
The present invention is directed to a method for cloning and producing the NotI restriction endonuclease by 1) introducing the restriction endonuclease gene from Nocardia otitidis-caviarum into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the plasmid encoding and expressing the NotI restriction endonuclease activity, and 3) purifying the NotI restriction endonuclease from the fermented host which contains the plasmid encoding and expressing the NotI restriction endonuclease activity.

REFERENCES:
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