Isolated DNA encoding the hphi restriction endonuclease and rela

Details Isolated DNA encoding the hphi restriction endonuclease and rela Isolated DNA encoding the hphi restriction endonuclease and rela

1995-07-21

1998-03-24

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

4352523, 4353201, 536 231, C12N 922, C12N 1555

Patent

active

057311854

ABSTRACT:
The present invention is directed to a method for cloning and producing the HphI restriction endonuclease by 1) linking up the restriction gene to transcription elements that can be processed in E. coli and 2) expressing DNA modification enzymes in the host that protect against HphI digestion.

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