Isolated DNA encoding the FSEI restriction endonuclease and rela

Details Isolated DNA encoding the FSEI restriction endonuclease and rela Isolated DNA encoding the FSEI restriction endonuclease and rela

1994-10-18

1996-08-06

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435199, 4353201, 43525233, 536 232, C12N 922, C12N 1555, C12N 1570

Patent

active

055433081

ABSTRACT:
The present invention is directed to a method for cloning and producing the FseI restriction endonuclease by 1) introducing the restriction endonuclease gene from Frankia species into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the plasmid encoding and expressing the FseI restriction endonuclease activity, and 3) purifying the FseI restriction endonuclease from the fermented host which contains the plasmid encoding and expressing the FseI restriction endonuclease activity.

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