Isolated CEL II endonuclease

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

Reexamination Certificate

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Reexamination Certificate

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07129075

ABSTRACT:
The present invention relates to the isolation and characterization of CEL I and CEL II endonuclease proteins. Methods and kits for identifying mismatches in double-stranded DNA are also provided.

REFERENCES:
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patent: 6699980 (2004-03-01), Yeung
Colbert et al., “High-Throughput Screening for Induced Point Mutations”, Plant Physiol. 2001 163:480-484.
Kulinski et al., “Modified Primers Facilitate Rapid Screening of 16S rRNA Gene Libraries”, BioTechniques 2000 29(1):44-48.
Oleykowski et al., “Mutation detection using a novel plant endonuclease”, Nucleic Acids Research 1998 26(20):4597-4602.
Sokurenko et al., “Detection of simple mutations and polymorphisms in large genomic regions”, Nucleic Acids Research 2001 29(22e111):1-8.
Yang et al., “Purification, Cloning and Characterization of the CEL 1 Nuclease”, Biochemistry 2000 39:3533-3541.

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