Isolated carotenoid biosynthesis gene cluster involved in...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing a carotene nucleus

Reexamination Certificate

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C435S069100, C435S320100, C435S252300, C435S254110, C435S325000, C435S419000, C536S023100, C536S023200, C536S023700, C530S350000

Reexamination Certificate

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10166037

ABSTRACT:
Isolated gene cluster involved in canthaxanthin biosynthesis, which comprises a polynucleotide wherein:crtY, crtI, crtB and crtW genes are clustered in this order and in the same orientation, and,preceding the four cited genes, crtE gene is oriented in the opposite direction.Applications for producing natural carotenoids useful in pharmaceutical, cosmetic and nutritious compositions.

REFERENCES:
patent: 5965795 (1999-10-01), Hirschberg et al.
Harker et al. Biosynthesis of ketocarotenoids in transgenic cyanobacteria expressing the algal gene for beta-C-4-oxygenase, crtO. FEBS Letters (1997) 404:129-134.
Mann et al. GenBank Accession No. CAB56040. phytoene desaturase [Nostoc sp. PCC 7120]. (1999).
Omura et al. GenBank Accession No. BAB69140. phytoene desaturase [Streptomyces avermitilis]. (2001).
Schumann et al. GenBank Accession No. AAA91950. phytoene desaturase. (1996).
Harker et al. GenBank Accession No. Y15112.Paracoccus marcusiicrtW, crtZ, crtY, crtI, crtB, & crtE genes. (1999).
Moluba et al., Applied and Environmental Microbiology, Jul. 1999, vol. 65, No. 7, pp. 3084-3094.
Lorquin et al., Applied and Environmental Microbiology, Mar. 1997, vol. 63, No. 3, pp. 1151-1154.
Hannibal et al, Journal of Bacteriology, Jul. 2000, pp. 3850-3853.

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