Isolated bacterial reverse transcriptase

Details Isolated bacterial reverse transcriptase Isolated bacterial reverse transcriptase

1989-02-24

1994-06-14

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

435822, 4352521, 935 14, C12N 912, C12N 112, C12N 100, C12N 1554

Patent

active

053209583

ABSTRACT:
The present invention relates to an isolated bacterial reverse transcriptase. The reverse transcriptase synthesizes a peculiar RNA-DNA complex called msDNA which is a single-stranded DNA structure branched out from an RNA molecule. The gene coding for the reverse transcriptase has been isolated and sequenced. It codes for a polypeptide of 485 amino acid residues. This is the first time that a reverse transcriptase has been found, identified and isolated from a prokaryotic microorganism of which the amino acid sequence is shown in FIGS. 2a-2d.

REFERENCES:
Dhundale et al, Structure of Ms DNA from My . . . , Cell, vol. 51, 1105-1112, 1987.
Dhundale et al, Mutations That Affect Production . . . , J. of Bacteriology, vol. 170, No. 12, 5620-5624, 1988.
Furuichi et al., Cell, 48, 47-53 (1987), "Branched RNA Covalently Linked to the 5' End of a Single-Stranded DNA in Stigmatella aurantiaca: Structure of msDNA".
Furuichi et al., Cell, 48, 55-62 (1987), "Biosynthesis and Structure of Stable Branched RNA Covalently Linked to the 5' End of Multicopy Single-Stranded DNA of Stigmatella aurantiaca".

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