Isolated agarase enzyme from flavobacterium SP. strain NR19, clo

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435200, 435207, 43525233, 4353201, 536 232, C12N 120, C12N 924, C12N 938, C12N 1500, C07H 2104

Patent

active

060016361

ABSTRACT:
Novel purified agarase enzymes from Flavobacterium sp. strain NR19 and cloned genes encoding the agarase enzymes are disclosed. Transformed host cells which express the novel agarase enzymes in isolatable quantities are also described. Also disclosed are antibodies specifically reactive with the novel agarases.

REFERENCES:
patent: 5418156 (1995-05-01), Stosz et al.
Sugano, Yasushi; Terada, Ichiro; Arita, Masatoshi; Noma, Masana; and Matsumoto, Takashi, Purification and Characterization of a New Agarase from a Marine Bacterium, Vibrio sp. Strain JT0107, Applied and Environmental Microbiology, (May 1993), pp. 1549-1554.
Sugano, Yasushi; Matsumoto, Takashi; Kodama, Hisashi; and Noma, Masana, Cloning and Sequencing of agaA, a Unique Agarase 0107 Gene from a Marine Bacterium, Vibrio sp. Strain JT0107, Applied and Environmental Microbiology. (Nov. 1993), pp. 3750-3756.
Sugano, Yasushi; Matsumoto, Takashi; and Noma, Masana, Sequence analysis of the agaB gene encoding a new .beta.-agarase from Vibrio sp. strain JT0107, Biochimica et Biophysica Acta, (1994), 1218 pp. 105-108.
Aoki, Takahiko; Araki, Toshiyoshi; and Kitamikado, Manabu; Purification and characterization of a novel .beta.-agarase from Vibrio sp. AP-2, Eur. J. Biochem., (1990), 187: 461-465.
Carlsson, J., and Malmqvist, M., Effects of Bacterial Agarase on Agarose Gel in Cell Culture, In Vitro, (1977), 13:7: 417-422.
Potin, Philippe; Richard, Christophe; Rochas, Cyrille; and Kloareg, Bernard, Purification and characterization of the .alpha.-agarase from Alteromonas agarlyticus (Cataldi) comb. nov., strain GJ1B, Eur. J. Biochem., (1993), 214: 599-607.
Young, K.; Hong, K.C.; Duckworth, M.; and Yaphe, W., Enzymic Hydrolysis of Agar and Properties of Bacterial Agarases, Proc. of the 7h International Seaweed Symp., (1971), pp. 469-472.
Leon, Oscar; Quintana, Luis; Peruzzo, Gina; and Slebe, Juan Carlos, Purification and Properties of an Extracellular Agarase from Alteromonas sp. Strain C-1, Applied and Environmental Microbiology, (Dec. 1992), pp. 4060-4063.
Andrykovitch, George and Marx, Irene, Isolation of a New Polysaccharide-Digesting Bacterium from a Salt Marsh, Applied and Environmental Microbilogy, (Apr. 1988), pp. 1061-1062.
Morrice, Lora M.; McLean, Maitland W.; Long, William F. and Williamson, Frank B., .beta.-Agarases I and II from Pseudomonas atlantica-Substrate specificities, Eur. J. Biochem. (1983) 137: 149-154.
Morrice, Lora M.; McLean, Maitland W.; Long, William F. and Williamson, Frank B., .beta.-Agarase I and II from Pseudomonas atlantica-Purifications and some properties, Eur. J. Biochem. (1983) 137: 149-154.
Groleau, D. and Yaphe, W., Enzymatic hydrolysis of agar: purification and characterization of .beta.-neoagarotetraose hydrolase from Pseudomonas atlantica, Can. J. Microbiol., (1977), 23: 672-679.
Belas, Robert; Bartlett, Douglas, and Silverman, Michael, Cloning and Gene Replacement Mutagenesis of a Pseudomonas atlantica Agarase Gene, Applied and Environmental Microbilogy, (Jan. 1988) 54:1, pp. 30-37.
Belas, Robert, Sequence Analysis of the agrA Gene Encoding .beta.-Agarase from Pseudomonas atlantica, Journal of Bacteriology, (Jan. 1989) pp. 602-605.
Malmqvist, Magnus, Purification and Characterization of Two Different Agarose-Degrading Enzymes, Biochimica et Biophysica Acta, (1978), 537: pp. 31-43.
Yamaura, Izumi; Matsumoto, Toshihiko; Funatsu, Masaru; Shigeiri, Hisaji and Shibata, Teruhiko, Purification and Some Properties of Agarase from Pseudomonas sp. PT-5, Agric. Biol. Chem., (1991) 55(10): pp. 2531-2536.
Bibb, Mervyn J.; Jones, George H.; Joseph, Richard; Buttner, Mark J. and Ward, Judy M., The Agarase Gene (dagA) of Streptomyces coelicolor A3(2): Affinity Purification and Characterization of the Cloned Gene Product, Journal of General Microbiology, (1987), 133: pp. 2089-2096.
Buttner, Mark J.; Fearnley, Ian M. and Bibb, Mervyn J., The agarase gene (dagA) of Streptomyces coelicolor A3(2): nucleotide sequence and transcriptional analysis, Mol Gen Genet, (1987), 209: pp. 101-109.
Usov, A.I. and Miroshnikova, L.I., Isolation of agarase from Littorina mandshurica by affinity chromatography on Biogel A, Carbohydrate Research, (1973), 43: pp. 204-207.
Gudz, S.P.; Matviiko, S.G. and Dumych, M.F., A New Species of Agarlytic Soil Bacteria of the Genus, Microbiologiya, (1986) 55(2): pp. 248-252.
Zobell, Claude E. and Allen, Esther C., The Significance of Marine Bacteria in the Fouling of Submerged Surfaces, J. Bacteriol., (1934), 29: PP. 239-251.
van der Meulen, H.J.; Harder, W. and Veldkamp, H., Isolation and characterization of Cytophaga flevensis sp. nov., a new agarolytic flexibacterium, Antonie van Leeuwenhoek, (1974), 40: pp. 329-346.
van der Meulen and Harder, W., Production and characterization of the agarase of Cytophaga flevensis, Antonie van Leeuwenhoek, (1975), 41: pp. 431-447.
van der Meulen, H.J. and Harder, W., Characterization of the neoagarotetra-ase and neoagarobiase of Cytophaga flevensis, Antonie van Leeuwenhoek, (1976), 42: pp. 81-94.
Duckworth, M. and Turvey, J.R., An Extracellular Agarase from a Cytophaga Species, Biochem. J., (1969), pp. 113, 139.
Kadokami, Yoichi and Lewis, Randolph V., Reverse Electrophoresis to Concentrate DNA Fractions, Analytical Biochemistry, (1995), 226: pp. 193-195.
Gnirke, Andreas; Huxley, Clare; Peterson, Ken and Olson, Maynard V., Microinjection of Intact 200- and 500-kb Fragments of YAC DNA into Mammalian Cells, (1993), Genomics, 15: pp. 659-667.
Potier, M.-C.; Kuo, W.L.; Dutriaux, A.; Gray, J. and Goedert, M., Construction and Characterization of a Yeast Artificial Chromosome Library Containing 1.5 Equivalents of Human Chromosome 21, (1992), Genomics, 14: pp. 481-483.
Maule, John C.; Porteous, David J. and Brookes, Anthony J., An improved method for recovering intact pulsed field gel purified DNA, of at least 1.6 megabases, (1994), Nucleic Acids Research, 22:15: pp. 3245-3246.
Gold, Paul, Use of a Novel Agarose Gel-Digesting Enzyme for Easy and Rapid Purification of PCR-Amplified DNA for Sequencing, (1992), BioTechniques 133, 13:1.
Carlsson, J. and Malmqvist, M., Effects of Bacterial Agarase Gel in Cell Culture, (1977), In Vitro, 13:7: pp. 417-422.
Gray, Forest; Kenney, John S. and Dunne, John F., Secretion capture and report web: use of affinity derivatized agarose microdroplets for the selection of hybridoma cells, (1995), Journal of Immunological Methods, 182: pp. 155-163.
Serwer, Philip; Hayes, Shirley J.; Moreno, Elena T. and Park, Christopher Y., A Small (58-nm) Attached Sphere Perturbs the Sieving of 40-80-Jilobase DNA in 0.2-2.5% Agarose Gels: Analysis of Bacteriophage T7 Capsid-DNA Complexes by Use of Pulsed Field Electrophoresis, (1992), Biochemistry, 31: pp. 8397-8405.
Hodgson, D.A. and Chater, K.F., A Chromosomal Locus Controlling Extracellular Agarase Production by Streptomyces coelicolor A3(2), and its inactivation by Chromosomal Integration of Plasmid SCP1, (1981), Journal of General Microbiology, 124: pp. 339-348.
Kendall, Kevin and Cullum, John, Cloning and expression of an extracellular-agarase gene from Streptomyces coelicolor A3(2) in Streptomyces lividans 66, (1984), Gene, 29: pp. 315-321.
Rochas, Cyrille; Potin, Philippe and Kloareg, Bernard, NMR spectroscopic investigation of agarose oligomers produced by an .alpha.-agarase, (1994), Carbohydrate Research, 253: pp. 69-77.
Hofsten, B.v. and Malmqvist, M., Degradation of Agar by a Gram-negative Bacterium, (1975), Journal of General Microbiology, 87: pp. 150-158.
Epicentre Technologies Publication, GELase.TM. Agarose Gel-Digesting Preparation Product Information, (Aug. 1993), 5 pages.
New England Biolabs Publication, .beta.-Agarase I, 3 pages.
Miscellaneous Biochemicals Publication, p. 263.
Alphabetical List of Compounds, Sigma, p. 54.
Calbiochem.RTM. Biochemicals for Research, 121814 Agarase, Pseudomonas atlantica, p. 8.
FMC Corporation Publication, .beta.-Agarase, 1 page.
Promega Technical Bulletin, Promega Corporation, Madison, Wisconsin, (Jun. 1995), PP. 1-17.
Knoche, Kimberly; Selman, Susanne; Kobs, Gary; Brady, Melinda and Knuth, Mark, Modifying Enzymes, (Oct. 1995), Promega Notes M

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Isolated agarase enzyme from flavobacterium SP. strain NR19, clo does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Isolated agarase enzyme from flavobacterium SP. strain NR19, clo, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Isolated agarase enzyme from flavobacterium SP. strain NR19, clo will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-862353

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.