Drug – bio-affecting and body treating compositions – Fermentate of unknown chemical structure
Reexamination Certificate
2000-10-19
2002-05-28
Lilling, Herbert J. (Department: 1651)
Drug, bio-affecting and body treating compositions
Fermentate of unknown chemical structure
C435S254100, C435S256800
Reexamination Certificate
active
06395271
ABSTRACT:
FIELD OF THE INVENTION
The present invention relates a process for culturing isolates of
Antrodia camphorata
to provide a product useful in medical and nourishment fields. The present invention also relates to a novel isolate of
Antrodia camphorata,
the culture of which exhibits pharmacological activities when the isolate is grown in a suitable medium.
DESCRIPTION OF THE RELATED ART
Antrodia camphorata
[(Zang & Su) S.-H. Wu, Ryvarden & T. T. Chang], also known as “niu-chang-chih” or “niu-chang-ku” in Taiwan, was recently reported as a new fungus species characterized by the cylindrical shape of its basidiospores appearing in fruit bodies, weakly amyloid skeletal hyphae, bitter taste and light cinnamon resupinate to pileate basidiocarps, as well as chlamydospores and anthroconidia in pure culture. The growth of this new fungus species is extremely slow and restricted to an endemic tree species,
Cinnamomum kanehirai
Hay (Lauraceae), as the only host. The detailed characterization and taxonomic position of
A. camphorata
were described in Chang, T. T. et al.,
Antrodia cinnamomea
sp. nov. on
Cinnamomum kanehirai
in Taiwan,
Mycol. Res.
99(6): 756-758 (1995) and Wu, S.-H., et al.,
Antrodia cinnamomea
(“niu-chang-chih”), New combination of a medicinal fungus in Taiwan,
Bot. Bull. Acad. Sin.
38: 273-275 (1997), the entire disclosure of which is incorporated herein by reference.
In Taiwanese folk medicine, the fruit bodies of
A. camphorate
are believed to have certain medical effects. According to the traditional way, the fruit bodies are ground into dry powder or stewed with other herbal drugs for oral uptake to treat conditions caused by poisoning, diarrhea, abdominal pain, hypertension, skin itches and liver cancer. However, no pharmacological or clinical study in these aspects has appeared in literature to date. Because of the stringent host specificity and rarity in nature, as well as the failure of artificial cultivation, “niu-chang-chih” is very expensive in Taiwan. In recent years, the fruit bodies of this fungus with high quality have been sold at an extremely high price of around U.S.$ 15,000 per kg.
Accordingly, there exists a need to establish a method for the large-scale cultivation of the fungus
A. camphorata
in a suitable artificial environment, while maintaining the pharmacological activities of the fungus
A. camphorata.
There also exists a need to obtain an isolate of
A. camphorata
that exhibits pharmaceutically useful activities when cultivated in a suitable artificial environment.
SUMMARY OF THE INVENTION
Accordingly, an object of the present invention is to provide a process suitable for culturing isolates of
A. camphorata,
which can grow well in the mycelial state when subjected to submerged fermentation. For this purpose, we have tested five isolates of
A. camphorata
collected in the Food Industry Research and Development Institute (FIRDI), Hsin-Chu, Taiwan, R.O.C. In addition, it is surprising to find that the culture of an obtained isolate exhibits a desired pharmacological activity, in particular an anti-tumor activity. According to a preferred embodiment of this invention, the carbon source of the medium, usually a carbohydrate, may be critical to obtain an optimized environment for culturing
A. camphorata.
Another object of the present invention is to provide a novel isolate of
Antrodia camphorata
bearing a desired pharmacological activity, in particular an anti-tumor activity, when grown in a suitable artificial environment. For this purpose, we have isolated and identified an isolate of
A. camphorata,
which can grow well in the mycelial state when subjected to submerged fermentation, and the resulting culture exhibits excellent pharmacological activities.
The present invention thus provides a process for producing a culture of an isolate of
A. camphorata
having pharmacological activity, comprising the steps of:
(a) inoculating a mycelial inoculum of an isolate of
A. camphorata
into a medium suitable for the growth of said isolate;
(b) cultivating the resulting culture from step (a); and
(c) harvesting the culture when the culture has a redness index a ≧3 as measured using Hunter's coordinate system.
A still another object of the present invention is to provide a product obtained from the above process.
A further object of the present invention is to provide a pharmaceutical composition or a nutrient supplement containing a product obtained from the above process.
REFERENCES:
Wu, S. H., Ryvarden, L. and Chang, T.T., 1997.Antrodia camphorata(“niu-chang-chih”), new combination of a medicinal fungus in Taiwan. Bot Bull. Acad. Sinica 38 : 273-275, Oct. 1997.
Chen Jian-Chyi
Huang Ren-Chang
Wang Bor-Cheh
Council of Agriculture Executive Yuan
Lilling Herbert J.
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