Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – 8 to 10 amino acid residues in defined sequence
Patent
1996-03-29
1999-02-02
Tsang, Cecilia J.
Chemistry: natural resins or derivatives; peptides or proteins;
Peptides of 3 to 100 amino acid residues
8 to 10 amino acid residues in defined sequence
530329, 530330, 530331, 530345, 204459, 930280, C07K 700
Patent
active
058666834
ABSTRACT:
Provided are isoelectric point(pI) markers for isoelectric focusing with fluorescence detection. The markers are fluorescence-labeled oligopeptides which comprise a fluorescence dye bonded chemically to the amino group of N-terminal amino acid of oligopeptide. The marker shows its unique and narrow pI band(peak) in electrophoresis or isoelectric focusing. The markers can be designed to have appropriate pI value, and cover wide range of pI (3<pI<11). Further, the markers have good storage stability. The markers can be preferably applied to the capillary isoelectric focusing with fluorescence detection due to their sharp and narrow band with strong emitted fluorescence.
REFERENCES:
patent: 5245551 (1993-09-01), Heron et al.
patent: 5348633 (1994-09-01), Karger et al.
patent: 5439829 (1995-08-01), Anderson et al.
Manabe, "Utilization of Microcomputer in Protein Chemistry", Chemical Field, 36:470-486 (1982).
Kai et al., "Fluorescence derivation of bioactive peptides for high-performance liquid chromatograpny", Trends in Analytical Chemistry, May, 1987, pp. 116-120.
Slais et al, "Low-molecular-mass pl markers for isoelectric focusing", Journal of Chromatography A, Feb., 1994, pp. 294-256.
Nashabeh et al, Electrophoresis vol. 14 407-16 (1993).
Kai et al., J. Chrom. A vol. 653 (1993) pp. 235-240.
Shimura et al., Electrophoresis vol. 16 pp. 1479-1484 (Aug. 1995).
Zhao et al., J. Chromat. vol. 608 (1992) pp. 239-242.
Dayhoff et al., Atlas of Protein Sequence and Structure 1972 vol. 5 pp. 89-99.
Kasai Kenichi
Matsumoto Hiroyuki
Shimura Kiyohito
Takamoto Hisayoshi
Celsa Bennett
Laboratory of Molecular Biophotonics
Tsang Cecilia J.
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