Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1982-07-27
1985-01-22
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 4, 435 18, 435195, 435199, 436 63, 436 86, C12Q 168, C12Q 100, C12Q 134, C12N 914, C12N 922, G01N 3350, G01N 2726
Patent
active
044952791
ABSTRACT:
An electrophoresis system which provides enhanced resolution and ability to identify nucleases through use of a two-dimensional technique involving isoelectric focussing of tube gels followed by the electrophoresis of second-dimension slab gels formed by the use of a holder allowing slab gels to be cast directly on the sides of the tube gels. DNA is employed as the substrate for casting the slab gels. The slab gels are electrophoresed for the second dimension in a chamber wherein the rack containing a stack of slab gels forms the partition between the anolyte and catholyte compartments. After the second-dimension electrophoresis, the slab gels are incubated in incubation buffer and placed in Pyronin Y to stain the unhydrolized DNA. After staining, they are destained in acetic acid. The DNAses are then visible as colorless spots in a reddish-colored gel.
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Anal. Biochem. 31, 506-511, (1969), Curtis W. Wilson, "A Rapid Staining Technique . . . ".
Plant Physiol., (1971), 48, 64-68, Curtis M. Wilson, "Plant Nucleases".
Febs Letters, vol. 51, No. 1, 266, Mar. 1975, L. C. Van Loon, "Polynucleotide-Acrylamide . . . ".
Anal. Biochemistry 58, 71-76, (1974), Zollner et al., "Human Serum Deoxyribonuclease . . . ".
Anal. Biochemistry 13, 28-42, (1965), Boyd et al., "Identification of Deoxyribonucleases . . . ".
Biochemistry, 1981, 20, 2261-2267, Blank et al., "Ribonucleases of Human Serum . . . ".
Brown Glenn E.
Karpetsky Timothy P.
Martinell James
The United States of America as represented by the Secretary of
Wiseman Thomas G.
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