Isoelectric focusing apparatus

Details Isoelectric focusing apparatus Isoelectric focusing apparatus

1986-03-20

1987-06-16

Niebling, John F.

Chemistry: electrical and wave energy

Apparatus

Coating, forming or etching by sputtering

2041823, 2041832, B01D 1302

Patent

active

046734830

ABSTRACT:
The disclosure is directed to an improved apparatus and method for isoelectric focusing whereby the reservoirs and recirculating paths in the apparatus are sealed off from the atmosphere. A liquid is employed which contains buffering compounds capable of maintaining a pH gradient in an electric field. A plurality of permeable diaphragms or membranes are used to separate the flows of liquid while allowing interchange of liquid constituents therebetween. A direct current electrical potential is established transverse to the flow, so as to maintain a gradient of pH steps as between successive channels defined by the membranes, and to separate a mixture of amphoteric substances into said channels.

REFERENCES:
patent: 4204929 (1980-05-01), Bier
patent: 4362612 (1982-12-01), Bier

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