Isoamylase gene, compositions containing it, and methods of usin

Details Isoamylase gene, compositions containing it, and methods of usin Isoamylase gene, compositions containing it, and methods of usin

1995-06-07

1998-05-12

Fox, David T.

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

The polynucleotide alters fat, fatty oil, ester-type wax, or...

800DIG42, 800DIG55, 800DIG56, 800DIG57, 800DIG58, 435 691, 435 697, 435 698, 435 701, 435101, 4351723, 435 98, 435194, 43525231, 43525233, 4352542, 435412, 435417, 435419, 536 232, 536 234, 536 237, A01H 500, C12N 1531, C12N 1554, C12N 1582, C12P 1904

Patent

active

057508763

ABSTRACT:
A method of producing plant products containing modified starch content, including higher ratios of amylose to amylopectin, increase in intermediate material, or amylopectin having fewer branches or altered branching pattern. Also provided are DNA constructs and transformed plant cells useful in that method. The preferred method uses isoamylase from a Flavobacterium sp., more preferably in combination with a gene encoding ADPglucose pyrophosphorylase. Also disclosed are the gene from Flavobacterium sp. and transformed bacterial and plant cells containing a derivative thereof.

REFERENCES:
patent: 5300145 (1994-04-01), Fergason et al.
Sato and Park (1980) Starch 32:Nr. 4,S.132-136 .vertline. Purification and Characterization of Extracellular Isoamylase from Flavobacterium sp.
Amemura et al. (1988) J. Biol. Chem. 263(19):9271-9275.
"Approaches to influence starch quantity and starch quality in transgenic plants" Muller-Rober B. and Kossman J., Plant, Cell and Environment, vol. 17, No. 5, 1994, pp. 601-613.
"Progress in the genetic manipulation of crops aimed at changing starch accumulation", Sivak M.N. and Preiss J., Databse Scisearch Institute for Scientific Information, Philadelphia, PA Genuine Article #:RU657, see abstract & J. Env. Polymer Degradation, vol. 3, No. 3, Jul. 1995, pp. 145-152.
Odibo, F.J.C. et al., World J. Microbiol. Biotechnol., 8:102-105 (1992).

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