Iron-releasing monokines

Details Iron-releasing monokines Iron-releasing monokines

1987-01-07

1992-07-07

Doll, John

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435 703, 435 704, 530351, C12N 500, C12P 2100, C07K 1500, C07K 1506

Patent

active

051282585

ABSTRACT:
A substantially purified preparation containing one or more soluble Iron-Releasing Monokines and a method of preparing and using same are disclosed. The monokine is heat labile and is retained by ultrafiltration on a YM-10 membrane. Molecular exclusion chromatography of macrophage conditioned supernatant containing the monokine yields fractions in the 30,000 to 65,000 relative molecular weight range with iron-releasing activity. Kinetic studies show that the monokine is rapidly released from activated macrophages after triggering with bacterial endotoxin, reaching plateau levels within 2-4 hours. The response of cells to the monokine depends on both the dose of the monokine administered and the duration of its exposure. The Iron-Releasing Monokine is distinct from other factors secreted by activated macrophages, such as a cytolytic factor and Respiratory Inhibition Factor which causes reversible lesions in the electron transport chain.

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