Iron colloid-labeled antibody, preparation thereof and histochem

Details Iron colloid-labeled antibody, preparation thereof and histochem Iron colloid-labeled antibody, preparation thereof and histochem

1988-09-09

1991-12-24

Rosen, Sam

Drug, bio-affecting and body treating compositions

Radionuclide or intended radionuclide containing; adjuvant...

Molecular bilayer structure

435 71, 435 721, 436501, 436547, 436548, 530387, 530388, G01N 130, G01N 3348

Patent

active

050751000

ABSTRACT:
Disclosed is an iron colloid-labeled antibody wherein an iron colloid is bound to IgG or an antigen binding fragment thereof without a loss of antibody specificity. An iron colloid stabilized with cacodylic acid or the salt thereof (ferric cacodylate colloid) is preferable.
This iron colloid-labeled antibody is prepared by mixing the iron colloid stabilized with cacodylic acid or the salt thereof with IgG or the antigen binding fragment thereof at a pH of about 7.4 to 7.5.
The iron colloid-labeled antibody is used for the histochemical detection of specific constituents of cells or tissued under both a light microscope and an electron microscope, which gives distinct stained figures in each case.

REFERENCES:
patent: 4252562 (1988-06-01), DeBrabunder et al.
Pares et al., Gold labelled Antibody Decoration in Diagnosis of Plant Virus by Immuno-Electro Microscopy, Journal of Immun. Methods, 51(1982) 23-28.
Moeremans et al., Ferr: Bye: Colloidal Iron Binding . . . for Staining of Proteins . . . Analytical Biochemistry 153, 18-22 (1986).
S. Seno et al., Catoionic Cacodylate Iron Colloid for Detection of Anionic Sites on Cell Surface and the Histochemical Stain of Acid Mucopolysaccharides, Histochemistry (1983) 78:27-31.
S. Seno et al., Fine-granular Cationic Iron Colloid--Its Preparation, Physicochemical Characteristics and Histochemical Use for the Detection of Ionized Anionic Groups, Histochemistry (1985) 82:307-312.

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