IRES enabled gene trapping in plants

Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...

Reexamination Certificate

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C435S419000, C435S320100, C800S278000, C800S295000

Reexamination Certificate

active

07348179

ABSTRACT:
Disclosed are methods for introducing nucleic acid constructs called “landing pads” in plant genes for the insertion of transgenes, and methods for introducing the transgenes into the landing pads. Transgenic plants and plant parts produced by the methods, and seeds derived from the plants, are also disclosed.

REFERENCES:
patent: WO-98/54342 (1998-12-01), None
patent: WO-98/55636 (1998-12-01), None
Kerbach et al. (TGG, 111: 1608-1616, 2005).
Urwin et al. (The Plant Journal, 24:583-589, 2000).
Coppoolse et al. (Plant Mol. Biol., 51:263-279, 2003).
Choi et al. (Nucleic Acids Research, 28:I-VII, 2000).
Skulachev, et. al. “Internal Initiation of Translation Directed by the 5′-Untranslated Region of the Tobamovirus Subgenomic RNA I(2)” Virology, 1999, vol. 263, pp. 139-154.
Ivanov, et. al. “A Tobamovirus Genome That Contains an Internal Ribosome Entry Site Functional in Vitro.” Virology, 1997, vol. 232, No. 1, pp. 32-43.
Toth, et. al. “A novel strategy for the expression of foreign genes from plant virus vectors.” FEBS Letters, Jan. 2001, vol. 489. No. 2-3. pp. 215-219.
Nathalie Hardouin and Andras Nagy; “Gene-Trap-Based Target Site for Cre-Mediated Transgenic Insertion”; INSERM, Genesis 26:245-252 Wiley-Liss (2000).

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