Electrolysis: processes – compositions used therein – and methods – Electrolytic analysis or testing – Of biological material
Reexamination Certificate
2005-03-25
2010-11-02
Nguyen, Nam X (Department: 1795)
Electrolysis: processes, compositions used therein, and methods
Electrolytic analysis or testing
Of biological material
C205S777500, C436S531000, C422S082030, C435S287200
Reexamination Certificate
active
07824539
ABSTRACT:
Ionic interactions are monitored to detect hybridization. The measurement may be done measuring the potential change in the solution with the ion sensitive electrode (which may be the conducting polymer (e.g., polyaniline) itself), without applying any external energy during the binding. The double helix formation during the complimentary hybridization makes this electrode act as an ion selective electrode—the nucleotide hydrogen bonding is specific and thus monitoring the ionic phosphate group addition becomes selective. Polyaniline on the surface of nylon film forms a positively charged polymer film. Thiol linkage can be utilized for polyaniline modification and thiol-modified single strand oligonucleotide chains can be added to polyaniline. The sensitivity is because the double helix formation during the complimentary hybridization makes this electrode act as an ion selective electrode as the nucleotide hydrogen bonding is specific and thus monitoring the ionic phosphate group addition becomes selective.
REFERENCES:
patent: 5491097 (1996-02-01), Ribi et al.
patent: 2002/0090649 (2002-07-01), Chan et al.
patent: 2003/0157527 (2003-08-01), Lastrucci
patent: 2005/0230271 (2005-10-01), Levon et al.
Kim, D.-S., et al. “Fabrication and Characterization of a Field Effect Transistor-Type Charge Sensor for Detecting Deoxyribonucleic Acid Sequence”, Japanese Journal of Applied Physics, Part I, vol. 42, No. 6B, Jun. 2003, p. 4111-4115.
Souteyrand, E., et al. “Direction Detection of the Hybridization of Synthetic Homo-Oligomer DNA Sequences by Field Effect”, Journal of Physical Chemistry, B, vol. 101, No. 15, no month given, 1997, p. 2980-2985.
Fritz, J., et al. “Electronic Detection of DNA by its Intrinsic Molecular Charge”, Proceedings of the National Academy of Science, USA, vol. 99, No. 22, Oct. 2002, p. 14142-14146.
Pouthas, F. et al. “DNA Detection on Transistor Arrays Following Mutation-Specific Enzymatic Amplification”, Applied Physics Letters, vol. 84, No. 9, Mar. 1, 2004, p. 1594-1596.
Janata, J. “Twenty Years of Ion-Selective Field-effect Transistors”, Analyst, vol. 119, Nov. 1994, p. 2275-2278.
Campanella, L., et al. “Sensitive Memberane ISFETs for Nitrate Analysis in Waters”, Sensors and Actuators, vol. 26-27, 1995, p. 329-335.
Campanella, L., et al. “New Polymeric Membrane ISFETs and ISEs Responsive to Cationic Surfactants”, 8th International Conference on Solid-State Sensors and Actuators and Eurosensors IX, Jun. 1995, p. 944-947.
Levon Kalle
Yu Bin
Zhou Yanxiu
Ball J. Christopher
Nguyen Nam X
Pokotylo John C.
Polytechnic Institute of New York University
Straub & Pokotylo
LandOfFree
Ionic based sensing for identifying genomic sequence... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Ionic based sensing for identifying genomic sequence..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Ionic based sensing for identifying genomic sequence... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4251613