Ionic attachment of biomolecules with a guanidino moiety to medi

Details Ionic attachment of biomolecules with a guanidino moiety to medi Ionic attachment of biomolecules with a guanidino moiety to medi

1998-01-22

1999-07-27

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

424422, 424 941, 435176, 435177, 435180, 435181, 436518, 436524, 436531, 436532, 530402, 530810, 530811, 530815, 530816, C12N 1100, G01N 33543, C07K 100, A61K 3843

Patent

active

059289163

ABSTRACT:
Methods are provided for forming a coating of an immobilized biomolecule on a surface of a medical device to impart improved biocompatibility for contacting tissue and bodily fluids. A biomolecule having a negatively charged moiety is combined with a medical device surface having a positively charged guanidino moiety to form an ionic bond immobilizing a coating of the biomolecule on the surface. In another method, the medical device surface contains an amine moiety that is combined with a guanidino forming agent to form a positively charged guanidino moiety that is combined with the negatively charged moiety to form the ionic bond. In a further embodiment, the medical device surface contains a negatively charged moiety, and a biomolecule containing an amine moiety is combined with a guanidino forming agent to form a positively charged guanidino moiety that is combined with the negatively charged moiety to form the ionic bond.

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