Ion-capture assays using a specific binding member conjugated to

Details Ion-capture assays using a specific binding member conjugated to Ion-capture assays using a specific binding member conjugated to

1994-01-27

1995-10-17

Scheiner, Toni R.

Chemistry: analytical and immunological testing

Involving immune complex formed in liquid phase

Separation of immune complex from unbound antigen or antibody

436539, 436541, 436544, 436528, 435 792, 435 793, 435 794, 435961, G01N 33544, G01N 33532, G01N 33538

Patent

active

054590809

ABSTRACT:
The present invention includes novel assays employing a capture reagent, involving a first specific binding member conjugated to a polymeric anion such as carboxymethylamylose, and a solid phase material containing a reaction site comprising a polymeric cation substance. A test sample suspected of containing the analyte of interest may be contacted with the capture reagent to form a charged capture reagent/analyte complex. The complex is then contacted to/ the oppositely charged solid phase to attract, attach, and immobilize the capture reagent/analyte complex. The use of carboxymethylamylose to prepare a suitably charged capture reagent provides a superior capture reagent that is capable of binding and retaining the analyte on the solid phase even in the presence of polyanionic non-specific binding blockers.

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