Inviable phages, their production and DNA thereof

Details Inviable phages, their production and DNA thereof Inviable phages, their production and DNA thereof

1992-09-15

1996-09-24

Martinell, James

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4352351, 4353201, 935 31, C12N 1500, C12N 1563

Patent

active

055590187

ABSTRACT:
Inviable T4 phage-like particles capable of directing the expression of large non-T4 DNA fragments from T4 expression control sequences are produced. Thus, E. coli harboring pBR322 derivatives containing cloned T4 gene 23 DNA sequences were infected with T4 phage carrying a deletion of the denB gene. Homology-dependent recombination results in the production of inviable phage-like particles containing DNA molecules composed of multiple, tandemly repeated copies of entire plasmid molecules covalently linked to single copies of normal phage genes. The yield of these inviable particles, intially low, was increased by means of a reiterated infection process that involves the use of a cloned T4 origin of replication. When T4 gene 32 expression control sequences linked in proper orientation to a DNA sequence coding for the non-T4 protein .beta.-galactosidase were also cloned in one such pBR322 derivative (pVH773), inviable phage particles capable of directing the synthesis of enzymatically active .beta.-galactosidase were produced. The present process is applicable to other T-even bacterio-phages.

REFERENCES:
patent: 4332897 (1982-06-01), Nakano et al.
patent: 4348478 (1982-09-01), Nakano et al.
patent: 4415660 (1983-11-01), Goldstein
patent: 4460689 (1984-07-01), Foor et al.
patent: 4845031 (1989-07-01), Shub et al.
Takahashi et al, Mol. Gen. Genet. 186: 497 (1982).
Blattner et al; Science 196: 161 (1977).
Bolivar et al; Gene 2: 95 (1977).
Christensen et al; in Bacteriophage T.sub.4 ; Christensen et al (ed.), 1983, American Socienty for Microbiology, Washington, D.C., pp. 184-188.
Geiduschek et al; in Bacteriophage T.sub.4 ; Chirstensen et al(ed.), 1983, American Society for Microbiology, Washington, D.C., pp. 189-192.
Casna et al; Gene 18: 297 (1982).
Mattson et al; J. Mol. Biol. 170: 343 (1983).
Mattson et al; J. Mol. Biol. 170: 357 (1983).
Young et al; J. Mol. Biol. 138: 423 (1980).
Maniatis et al, Molecular Cloning, A Laboratory Manual, 1982, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y., pp. 76-78.

Affiliated with

Also associated with

Rating

Inviable phages, their production and DNA thereof does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Inviable phages, their production and DNA thereof, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Inviable phages, their production and DNA thereof will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1927992