Inverted latency specific binding assay

Details Inverted latency specific binding assay Inverted latency specific binding assay

1983-09-01

1987-12-15

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 7, 435 8, 435 14, 435 25, 435 28, 436520, 436522, 436537, 436546, 436829, C12Q 100, G01N 3353

Patent

active

047133244

ABSTRACT:
A specific binding assay composition and method for determining a ligand in a sample are disclosed. The composition comprises (a) a binding partner for the ligand; (b) a detection system which has at least two components; (c) a selectively accessible vesicle having a surface-incorporated ligand or ligand analog and a first component of the detection system therein; (d) a substance which modifies vesicle accessibility in response to binding of surface-associated ligand or ligand analog and binding partner; and (e) at least one additional component of the detection system which is reactive with the first component to produce a detectable response which is reduced by association of the binding partner and vesicle modifying substance with the variabily accessible vesicle. A decrease in signal is measured upon reaction as compared to the signal of unreacted vesicles.

REFERENCES:
patent: 4134792 (1979-01-01), Boguslaski et al.
patent: 4174384 (1979-11-01), Ullman et al.
patent: 4193983 (1980-03-01), Ullman et al.
patent: 4220450 (1980-09-01), Maggio
patent: 4235792 (1980-12-01), Hsia et al.
patent: 4342826 (1982-08-01), Cole
patent: 4372745 (1983-02-01), Mandle et al.
Smolarsky, et al., J. Immunol. Meth., vol. 15, (1977), pp. 255-265.
Geiger, et al., J. Immunol. Meth., vol. 17, (1977).
Haga, et al., Biochem. Biophys. Res. Comm., vol. 95, (1980), pp. 187-192.
Magee, et al., J. Cell Biology, vol. 63, (1974), pp. 492-504.
Six et al., Biochemistry, vol. 13, (No. 19), pp. 4050-4058, (1974).

Affiliated with

Also associated with

Rating

Inverted latency specific binding assay does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Inverted latency specific binding assay, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Inverted latency specific binding assay will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1219952