Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1993-08-20
1996-11-19
Moody, Patricia R.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536 236, 435 691, 4351723, 935 35, C07H 2104, C12N 1511, C12N 1529, C12N 1582
Patent
active
055764282
ABSTRACT:
Transgenic plants that are modified to produce fruits that have altered levels of soluble solids compared to non-transgenic plants of the same species are provided. The transgenic plants are prepared by introducing into plants DNA constructs that encode invertase operatively linked to DNA encoding regulatory regions that direct transcription of the DNA encoding invertase and operatively linked to DNA encoding amino acids that direct proper processing of the invertase through the secretory pathways of the plant and targeting of the invertase to the vacuole.
In particular, DNA constructs encoding tomato plant vacuolar invertase in operative linkage with a developmentally regulated promoter region are provided. Preferred regulatory and structural DNA is obtained from genomic DNA clones and cDNA clones encoding tomato fruit vacuolar invertases from the commercial tomato plant, Lycopersicon esculentum, and wild tomato plant, Lycopersicon pimpinellifolium.
Probes derived from the genomic DNA and cDNA, antibodies specific for tomato fruit invertase, and uses therefor, are also provided.
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Butler William O.
Dickinson Craig D.
Elliott Kathryn J.
Fitzmaurice Leona C.
Konno Yoshihiro
Moody Patricia R.
The Salk Institute Biotechnology Industrial Associates, Inc.
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