Inulin synthase and process for producing inulin by using...

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease

Reexamination Certificate

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C435S004000, C435S006120, C435S069100, C435S183000, C435S252300, C435S252500, C435S320100, C536S023200, C536S023500, C536S023600, C536S023700

Reexamination Certificate

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10311318

ABSTRACT:
The present invention relates to a novel inulin synthase having a function and a substrate specificity of acting on sucrose to produce inulin, but not acting on kestose, maltose, lactose, trehalose and cellobiose; and a process for producing inulin comprising the step of allowing the synthase, a culture fluid or cultured cells of a microorganism producing the synthase, or a treated product thereof to contact with sucrose to produce inulin.

REFERENCES:
patent: 5952205 (1999-09-01), Catani et al.
patent: 6242225 (2001-06-01), Catani et al.
patent: 6423833 (2002-07-01), Catani et al.
patent: WO 00/31287 (2000-06-01), None
Koops et al. (Plant Physiol., 1996, vol. 110(4):1167-1175).
T. Wada, et al., Bioscience, Biotechnology, and Biochemistry, vol. 67, No. 6, XP-002277857, pp. 1327-1334, “A Novel Enzyme ofBacillussp. 217C-11 That Produces Inulin from Sucrose”, Jun. 2003.
S.A.F.T. Van Hijum, et al., Applied and Environmental Microbiology, vol. 65, XP-002264246, pp. 73-96, “Molecular Characterization of a Novel Fructosyltransferase FromLactobacillus reuteriSynthesizing a High Molecular Weight Fructan with β-(2→ 1) Linked Fructosyl Units inEscherichia coli”, 1999.
B.W. Kim et al.: “Selective production of GF4-fructooligosaccharide from sucrose by a new transfructosylating enzyme” Biotechnol. Lett., vol. 20, No. 11, pp. 1031-1034.
E.M. Hellwage et al.: “Difference in chain length distribution of inulin fromCynara scolymusandHelianthus tuberosusare reflected in a transient plant expression system using the respective 1-FFT cDNAs” FEBS Lett., vol. 427, pp. 25-28 1998.

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