Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1989-03-31
1991-08-27
Martinell, James
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4351723, 4352401, 4353201, 536 27, 935 34, 935 61, 935 66, 935 70, 935 71, 935 79, 935 84, C12N 100, C12N 1512, C12N 1511, C12N 500
Patent
active
050432702
ABSTRACT:
DNA constructs are provided employing intronically positioned expression, systems, where one of the genes is a dominant gene, usually amplifiable, and the other gene encodes a sequence of interest. Higher levels of co-expression are achieved than when the genese are ligated in tandem. Specifically, the gene of interest is inserted into the intron of a DHFR minigene, the construct transformed into a mammalian cell and the resulting transformants stressed with progressively increasing levels of methotrexate. Substantially increasing levels of co-expression are achieved with increasing levels of methotrexate.
REFERENCES:
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"Molecular Cloning of the neu Gene: Absence of Gross Structural Alteration in Oncogenic Alleles" Hung et al., (1986) Proc. Natl. Acad. Sci. USA, 83: 261-264.
"Gene Within a Gene: Nested Drosophila Genes Encode Unrelated Proteins on Opposite DNA Strands" Henikoff et al., (1986) Cell, 44: 33-42.
"Methotrexate-Induced Amplification of the Bovine Lutropin Genes in Chinese Hamster Ovary Cells" Kaetzel et al., (1988) Journal of Biological Chemistry, 263: 6344-6351.
"Coamplification and Coexpression of Human Tissue-Type Plasminogen Activator and Murine Dihydrofolate Reductase Sequences in Chinese Hamster Ovary Cells" Kaufman et al. (1985) Molecular and Cellular Biology, 5: 1750-1759.
"Competitive Inhibition of hsp70 Gene Expression Causes Thermosensitivity" Johnston et al., (1988) Science, 242: 1551-1554.
"Amplification and Expression of Sequences Cotransfected with a Modular Dihydrofolate Reductase Complementary DNA Gene" Kaufman et al., (1982) Journal of Molecular Biology, 159: 601-621.
Abrams John M.
Schimke Robert T.
Thorpe Susan M.
Martinell James
Rowland Bertram I.
The Board of Trustees of the Leland Stanford Junior University
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