Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1990-07-11
1993-03-09
Moskowitz, Margaret
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 91, 935 17, 935 18, 935 76, 935 77, 935 78, C12Q 168, C12P 1934
Patent
active
051926598
ABSTRACT:
The present invention provides a method for detection of at least one allele of a genetic locus and can be used to provide direct determination of the haplotype. The method comprises amplifying genomic DNA with a primer pair that spans an intron sequence and defines a DNA sequence in genetic linkage with an allele to be detected. The primer-defined DNA sequence contains a sufficient number of intron sequence nucleotides to characterize the allele. Genomic DNA is amplified to produce an amplified DNA sequence characteristic of the allele. The amplified DNA sequence is analyzed to detect the presence of a genetic variation in the amplified DNA sequence such as a change in the length of the sequence, gain or loss of a restriction site or substitution of a nucleotide. The variation is characteristic of the allele to be detected and can be used to detect remote alleles. Kits comprising one or more of the reagents used in the method are also described.
REFERENCES:
patent: 4582788 (1986-04-01), Erlich
patent: 4683194 (1987-07-01), Saiki
patent: 4683202 (1987-07-01), Mullis
DiLella et al., Nature, 322, 799-803 (1986).
DiLella et al., The Lancet, Mar. 5, 1988 (1988).
Maeda et al., Tissue Antigens, (1989), 34:290-298.
Geisel et al., Abstract Biosis No. 87082065.
J. Clin. Chem. Clin. Biochem., 26(7), 1988, 429-434.
Funke et al., Biosis No. 84025060 Abstract.
J. Clin. Chem. Clin. Biochem., 25(3), 1987, 131-134.
Kerem et al. Science, 245:1073-1080 (1989).
Deng, Nucleic Acids Research, 16(13):(6231).
Graham et al., Blood, 66, 759-764 (1985).
Saiki et al., Science, 230: 1350-1354 (1985).
Funke et al., J. Clin. Chem. Clin. Biochem., 25, 131-134 (1987).
Molecular Biology of the Gene, Fourth Edition, Calif., 1987, Chapter 20: "Conserved Sequences at Exon-Intron Boundaries in Pre-mRNA", pp. 639-640.
Clark, Mol. Biol. Evol., 7(2):111-122 (1990).
Dicker et al., BioTechniques, 7:830-837 (1989).
DiLella et al., The Lancet, i:497-499 (1988).
Kogan et al., New Engl. J. Med., 317:985-990 (1987).
Mardis et al., BioTechniques, 7:840-850 (1989).
Marx, Science, 247:1540-1542 (1990).
Riordan et al., Science, 245:1066-1072 (1989).
Rommens et al., Science, 245:1059-1065 (1989).
Simons et al., pp. 952-958 In: Immunology of HLA, vol. 1:Springer-Verlag, New York (1989).
Stephens et al., Am. J. Hum. Genet., 46:1149-1155 (1990).
Molecular Biology of the Cell, Second Edition, New York, 1989, Chapter 9: "RNA Synthesis and RNA Processing", pp. 531-535 and Chapter 10: Control of Gene Expression, pp. 602-604.
Erlich et al., J. Forensic Sciences, Sep.:1017-1018 (1990).
GeneType AG
Moskowitz Margaret
Terlizzi Laura
Yuan Lori L.
LandOfFree
Intron sequence analysis method for detection of adjacent and re does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Intron sequence analysis method for detection of adjacent and re, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Intron sequence analysis method for detection of adjacent and re will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-210615