Introduction of heterologous genes into bacteria using transposo

Details Introduction of heterologous genes into bacteria using transposo Introduction of heterologous genes into bacteria using transposo

1989-05-26

1992-04-07

Lacey, David L.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4353201, C12N 1563, C12N 1590

Patent

active

051027976

ABSTRACT:
This invention relates to a new method for inserting heterologous genes into the genome of a bacteria using a combined plasmid. The combined plasmid provides a cis complementation of transposase genes and transposable elements. The method involves the homologous recombination of a carrier plasmid and a functions plasmid to form the combined plasmid. The carrier plasmid contains a transposable element which flanks a generic expression cassette. The functions plasmid comprises transposase genes which complement the transposable element on the carrier plasmid. The combined plasmid is then transferred to a recipient and the recipient is monitored for integration of the generic expression cassette into the genome. The combined plasmid is preferably created by an in vivo homologous recombination of the carrier and functions plasmids.

REFERENCES:
patent: 4940838 (1990-04-01), Schilperoort et al.
Mol. Gen. Genet 213:332-338, 1988, Elliott and Roth, Characterization of Tn 10d-Cam: A Transposition-Defective Tn10 Specifying Chloramphenical Resistance.
Gene 51:91-96, 1987, Obukowicz et al., ISSOL as a Non-Self Transposable Vector Used to Integrate the Bacillus thuringiensis delta-encotoxin gene into . . . .
Biotechnology 4:446-449, May 1986, Bart et al., Permanent Insertion of Foreign Genes into the Chromosomes of Soil Bacteria.

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