Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1990-07-26
1993-08-03
Brown, Johnnie R.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 4, 435 71, 435 14, 435 21, 435 28, 435800, 436501, 436588, 536 231, C12Q 168, C12Q 125, C12Q 142, C12Q 128
Patent
active
052328309
ABSTRACT:
Novel methods and compositions for detecting a member of a ligand pair on solid supports having intrinsic fluorescence are disclosed. A target member of a ligand pair is contacted with a capture member of a ligand pair, wherein the capture member is immobilized on a solid support having intrinsic fluorescence, and the contacted pair is in association with a colorimetric reporter, then the solid support is irradiated the solid support, and the resultant fluorescence is determined. Preferably, methods include hybridization assays of nucleic acid sequences on such solid supports. The extent of detecting a member of a ligand pair, preferably a nucleic acid sequence, is determined using a method or technique described throughout this document as fluorescent quenching. Methods and compositions pertaining to solid supports having their intrinsic or natural fluorescence quenched or masked are also described herein. The present invention has utility in detection assays for a member of a ligand pair. Such ligand pairs include, but are not limited to, antigens (or epitopes) and appropriate antibodies, complementary nucleic acid sequences, hormones and their receptors, enzymes and corresponding inhibitors, lectins and sugars, etc.
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Brown Johnnie R.
Kunz Gary L.
Microprobe Corporation
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