Intrinsic factor - horse peroxidase conjugates and a method for

Details Intrinsic factor - horse peroxidase conjugates and a method for Intrinsic factor - horse peroxidase conjugates and a method for

1992-09-04

1994-09-27

Ceperley, Mary E.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435188, 435962, 435964, 435975, G01N 33566, C12N 996

Patent

active

053506740

ABSTRACT:
This invention presents a non-isotopic competitive assay for Vitamin B12 (B12), utilizing intrinsic factor (IF) labelled with horse radish peroxidase (HRP), by coupling via heterobifunctional cross-linking agents. In addition, a method for stabilizing the resultant conjugates by pretreatment with N-ethylmaleimide is disclosed.

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