Intrinsic enzyme dot blot immunoassay or indentification of micr

Details Intrinsic enzyme dot blot immunoassay or indentification of micr Intrinsic enzyme dot blot immunoassay or indentification of micr

1988-08-18

1991-10-22

Saunders, David

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 732, 435 74, 435967, 435970, 435973, 435975, 436518, 436530, 436809, G01N 33543, G01N 33548, G01N 33569

Patent

active

050595229

ABSTRACT:
A method for performing an assay to differentiate between related taxa of micoorganisms, comprising the steps of providing a support having a plurality of zones, each zone comprising immobilized antibody to an enzyme found in a particular microorganism, wherein the antibody in the zone is specific to the enzyme, but not to corresponding enzyme of other taxa to be determined in the assay; contacting the zones with a sample suspected of containing one of the particular microorganisms; permitting enzyme in the sample to become bound by antibody in one or more of the zones; and determining which of the taxa were present in the sample by determining to which of the zones the enzyme has become bound. The determining step may comprise adding a substrate for the enzyme to the support after the enzyme has become bound thereto, wherein the substrate, after being acted on by the enzyme, creates a detectable signal. Also disclosed are an assay kit, an immunoreactive support, and a method for preparing the support, all for use in the method of the present invention.

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