Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Patent
1993-04-16
1995-12-12
Knode, Marian C.
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
435853, 435856, 435857, 424 9345, C12N 120
Patent
active
054749328
DESCRIPTION:
BRIEF SUMMARY
This application is a 371 of PCT/SE92/00528, filed Jul. 24, 1992.
The present invention refers to a process for isolation of strains of Lactobacillus having the ability to colonize and become established on intestinal mucosa in vivo after oral administration, strains obtained by this process, and the use thereof for the prophylaxis or treatment of bacterial infections, especially in the form of a composition comprising an oatmeal based nutrient solution fermented by one of said strains.
Many people have a disturbed intestinal microflora, that is, the balance between useful and harmful intestinal bacteria is disturbed. A number of factors, among others stress, the occurence of bile salts, diet, etc. influence the bacterial flora. Most important is, however, that modern antibiotic treatment can destroy the normal flora for a long period of time, and thus, eliminate a normal fermentation process. Should the fermentation process be disturbed and the number of useful bacteria be reduced, the consequence will be that the colon mucosa withers away and ceases to function at the same time as the potentially malignant bacteria rapidly grow in number. These bacteria penetrate the malfunctioning colon wall and infect the organs of the body which leads to the so called intensive-care-disease with pus foci all over the body and possibly also an abolished function of most of the organs of the body, a collapse of organs. Blood poisoning, sepsis, caused by abscesses in the abdominal cavity is still a very common surgical complication in connection with abdominal surgery with a high death-rate. These patients are today treated by administration of antibiotics and surgical treatment of the abscess to the extent it could be located. At present antibiotics are conventionally administered before intestinal surgery in order to reduce the risk of post-operative infections and illness caused thereby. However, the treatment with antibiotics is expensive and moreover associated with a risk of different complications such as allergy and destruction of the normal intestinal flora and overgrowth with still more pathogenic bacteria.
The fact that lactobacilli should have a favourable effect on the intestinal mucosa is an old idea which has been brought up again. There are however many unclear points as to which microorganisms are involved and as to the ecology of the intestines. Another problem in this connection is that the classification of the genus Lactobacillus is incomplete which makes it difficult to identify those strains which are favourable to the function of the intestines. What, after all, seems to be commonly accepted today is that: the establishing of pathogenic bacteria in various ways, irrespective of foodstuffs or intestines being concerned; the same species in protecting and activating the intestines; reducing effect, probably because of a checking of the cholesterol production in the intestines, but maybe also because the bacteria use cholesterol for the production of steroids; motoric activity, the cause of this effect is unknown; something which seems to have several grounds. Firstly, certain lactobacilli are able to prevent the production of nitroseamines in the intestines by means of the enzyme nitritereductase; nitroseamines are cancerogenic. Secondly, lactobacilli may obstruct certain bacterially produced enzymes in the intestines from activating potentially carcinogenic substances. Finally, there are indications of lactobacilli having growth restricting effect on cancer rumours, maybe because the macrophages of the immunological defence system are activated by the presence of the lactobacilli.
A decisive weakness of the lactobacilli used today in most conventional foodstuffs is the poor survival of these organisms during the passage through the stomach and duodenum. This brought about a the development of a product called "acidofilusfil", acidophilus sourmilk, wherein milk was fermented with a strain of Lactobacillus acidophilus isolated directly from human faeces. L. acidophilus manages the passage through the
REFERENCES:
Savage, Prog. Food Natr. Sci 7(3-4):65-74 1983 Abstract Medline 84096420.
International Journal of Systematic Bacteriology, vol. 40, No. 2, Apr. 1990, Marie Stahl,. et al., "Restriction Endonuclease Patterns and Multivariate Analysis as a Classification Tool for Lactobacillus spp.", pp. 189-192.
System, Appl. Microbiol., vol. 11, 1989, Siv Ahrne, et al., "Plasmids in Lactobacillus Strains Isolated Form Meat and Meat Products", 320-324.
Scand J. Infect Dis, vol. 4, 1987, Ann Lidbeck, et al., "Impact of Lactobacillus Acidophilus Supplements on the Human Oropharyngeal and Intestinal Microflora", pp. 1-7.
Ahrne Siv
Bengmark Stig
Jeppson Bengt
Molin Goran
Knode Marian C.
Probi AB
Witz Jean C.
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