Internal positive controls for nucleic acid amplification

Details Internal positive controls for nucleic acid amplification Internal positive controls for nucleic acid amplification

1995-06-07

1999-03-16

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 5, 435 912, 536 243, 536 2432, 536 2433, 536 231, C12Q 168, C12P 1934, C07H 2104, C07H 2102

Patent

active

058828575

ABSTRACT:
The present invention relates to an improvement in a method for amplifying a target sequence of a target polynucleotide. The method comprises combining a sample suspected of containing the target polynucleotide with reagents for amplifying the target sequence and subjecting the combination to conditions wherein the target sequence if present is amplified. The present improvement comprises including in the combination a control oligonucleotide and a control polynucleotide that has a sequence that is hybridizable with the control oligonucleotide. When the control oligonucleotide is bound to the control polynucleotide, the ability of a primer to chain extend along the control polynucleotide is reduced. Optionally, the control oligonucleotide is part of the control polynucleotide. The method finds particular application in the area of nucleic acid amplification and detection.

REFERENCES:
patent: 5091310 (1992-02-01), Innis
patent: 5314809 (1994-05-01), Erlich et al.
patent: 5334515 (1994-08-01), Rastichian et al.
patent: 5340728 (1994-08-01), Grosz et al.
patent: 5348853 (1994-09-01), Wang et al.
patent: 5411875 (1995-05-01), Jones
Launer et al. "Simple method for cDNA amplification starting from small amount of total RNA" Molekuliarnia Genetika Mikrobiologia, I Virusolonga 6: 38-41, 1994.
Becker Andre et al. NAR 17: 9437-9446, 1989.
Gilliand et al. PNAS 87: 2725-2729, 1990.
Ferre, PCR Methods and Applications 2: 1-9, 1992.

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