Internal control for immunocytochemistry assay

Details Internal control for immunocytochemistry assay Internal control for immunocytochemistry assay

1993-06-08

1997-03-11

Scheiner, Toni R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 721, 435960, 435967, 436518, 436 63, 436 64, G01N 3353, G01N 33574

Patent

active

056100221

ABSTRACT:
An internal control or standard is provided for the direct quantitative assay by immunocytochemistry of target molecules in tissue specimens and the like. The control may be subjected to the same conditions including immunostaining as the tissue specimen. Immunoreactivity of the control and the specimen before and after processing is compared by physical measurement, e.g., optical density as determined by a cell analysis computer system. The immunoreactivity difference of the control provides a quantative standard useful to determine the effect of the processing on the immunoreactivity of the specimen.

REFERENCES:
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Dorland's Illustrated Medical Dictionary, 26th Edition, edited by Saunders, W.B. Saunders Co., pp. 1130-1131, 1985.
Walker, et al., The Journal of Urology, vol. 142, No. 6, pp. 1578-1583, Dec. 1989.
Histology, Cell and Tissue Biology, Fifth Edition, edited by L. Weiss, Elsevier Biomedical, NY, 1983, pp. 88-90 & 104-107.
Vartdal, F., et al., J. Immunol. Methods, 92, pp. 125-129, 1986.
Nabors, Journal of Neuroscience Methods 26:25-34 (1988).

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