Intermittent immersion vessel apparatus and process for...

Chemistry: molecular biology and microbiology – Apparatus – Means for separation or recovery of a microorganism from...

Reexamination Certificate

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C435S410000, C435S420000, C435S431000

Reexamination Certificate

active

07015032

ABSTRACT:
A plant micropropagation apparatus and process is provided in which a support platform for vessels containing a liquid growth media are periodically pivoted which brings about an intermittent immersion of the plant tissue/growth substrate in the growth media. The motion of the support platform may optionally be used to engage a piston operated dispenser for supplying aseptic media to the sealed vessel. The intermittent immersion also provides for an improved method of separating viable embryos from culture materials using a separation matrix in conjunction with the intermittent wave motion of the plant media when suspended in a liquid culture.

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patent: WO 0134807 (2001-05-01), None
International Search Report, PCT/US01/06586, 4 pages Aug. 6, 2001, Washington, DC.
International Preliminary Examination Report, PCT/US01/06586, 6 pages Feb. 1, 2002, Washington, DC.
Harris and Mason, “Two Machines For In Vitro Propagation of Plants in Liquid Media”, Canadian Journal of Plant Science, 63:311-316.

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