Chemistry: analytical and immunological testing – Biospecific ligand binding assay
Patent
1986-06-17
1990-05-15
Warden, Robert J.
Chemistry: analytical and immunological testing
Biospecific ligand binding assay
436500, 436504, 436517, 436536, 436537, 436172, 436805, 436518, 435 6, G01N 33566, G01N 2176
Patent
active
049258044
ABSTRACT:
A new reporter mechanism for biospecific reactions is disclosed. This mechanism involves interligand metal ion transfer in which a metal ion is directly transferred from one chelate complex to another following the occurance of the biospecific reaction. The second chelate complex is separate from and detectably different than the first chelate complex. This invention can take the form of methods of chemical analysis and kits for conducting such methods. In preferred embodiments of this invention the detectable difference is a difference in fluorescence, such as an increase or decrease which occurs as a result of the formation of the second chelate. In further preferred embodiments the difference in fluorescence is detected using fluorescent background rejection methods.
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Hale Ron L.
Wieder Irwin
Baxter International Inc.
Scheiner Toni R.
Warden Robert J.
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