Interligand metal transfer assay

Chemistry: analytical and immunological testing – Biospecific ligand binding assay

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436500, 436504, 436517, 436536, 436537, 436172, 436805, 436518, 435 6, G01N 33566, G01N 2176

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active

049258044

ABSTRACT:
A new reporter mechanism for biospecific reactions is disclosed. This mechanism involves interligand metal ion transfer in which a metal ion is directly transferred from one chelate complex to another following the occurance of the biospecific reaction. The second chelate complex is separate from and detectably different than the first chelate complex. This invention can take the form of methods of chemical analysis and kits for conducting such methods. In preferred embodiments of this invention the detectable difference is a difference in fluorescence, such as an increase or decrease which occurs as a result of the formation of the second chelate. In further preferred embodiments the difference in fluorescence is detected using fluorescent background rejection methods.

REFERENCES:
patent: 3901654 (1975-08-01), Gross
patent: 3939350 (1976-02-01), Kronick et al.
patent: 4020151 (1977-04-01), Bolz et al.
patent: 4058732 (1977-11-01), Wieder
patent: 4150295 (1979-04-01), Wieder
patent: 4220450 (1980-09-01), Maggio
patent: 4283382 (1981-08-01), Frank et al.
patent: 4341957 (1982-06-01), Wieder
patent: 4352751 (1982-10-01), Wieder et al.
patent: 4374120 (1988-02-01), Soini et al.
patent: 4565790 (1985-01-01), Hemmila et al.
Wieder, I., 1978 Elsevier/North Holland Biomedical Press Immunofluorescence and Related Staining Techniques W. Knapp, K. Holubar and G. Wick eds.
Hemmila, I., et al., Analytical Biochemistry 137 335 (1984)--Europium as a Label in Time Resolved Immunofluorometric Assays.
Soini, E., et al., Clin. Chem. 29/1, 65-68 (1983) Time Resolved Fluorometer for Lathanide Chelates--A New Generation of Nonisotopic Immunoassays.
Malmivuo, J., et al., Proceedings of the Fourth National Meeting on Biophysics and Medical Engineering and the Third National Meeting in Industry Tampere Finland.
Ann Clin. Biochem. 1981 18 253-274 A review of fluoroimmunoassay and immunofluorometric assay D. S. Smith M. Al-Hakiem and J. Landon.

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