Interleukin-2 signal transducers and binding assays

Details Interleukin-2 signal transducers and binding assays Interleukin-2 signal transducers and binding assays

1995-02-23

1997-04-08

Fleisher, Mindy

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4353201, 43525233, 435325, 435372, 536 235, C12P 2106, C12N 500, C12N 1512, C12N 1563

Patent

active

056186932

ABSTRACT:
The invention provides methods and compositions relating to interleukin 2 signal transducers, particularly an isolated human signal transducer and activator of transcription 5 (hStat 5), or a fragment thereof having an hStat 5-specific binding affinity, nucleic acids encoding hStat 5, which nucleic acids may be part of hStat 5-expression vectors and may be incorporated into a recombinant cell, agents which selectively bind hStat 5 or hStat 5 intracellular binding targets, or disrupt the binding of hStat 5 to such intracellular targets, methods of making such agents and hStat 5-specific binding targets in the form of cell surface proteins and nucleic acids. An hStat 5 drug screening assay involves forming mixtures of an hStat 5, an intracellular hStat 5 binding target, and a prospective agent at different concentrations. The mixtures are incubated to permit the binding of the intracellular hStat 5 binding target to the hStat 5 and the mixtures are then analyzed for the presence of such binding. A difference in such binding between the first and second mixtures indicates that the agent is capable of modulating the binding of hStat 5 to an intracellular hStat 5 binding target.

REFERENCES:
Beadling et al., "Activation of JAK Kinases and STAT Proteins by Interleukin-2 and Interferon .alpha., but not the T Cell Antigen Receptor, in human T Lymphocytes", The EMBO Journal 13(23):5605-5615 (1994).
Gilmour and Reich, "Receptor to Nucleus Signaling by Prolactin and Interleukin 2 via Activation of Latent DNA-binding Factors", Proc. Natl. Acad. Sci. USA 91:6850-6854 (1994).
Wakao et al., "Mammary Gland Factor (MGF) is a Novel Member of the Cytokine Regulated Transcription Factor Gene Family and Confers the Prolactin Response" The EMBO Journal 13(9):2182-2191 (1994).

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