Interleukin-2 receptor subunit ectodomain fusion protein compris

Details Interleukin-2 receptor subunit ectodomain fusion protein compris Interleukin-2 receptor subunit ectodomain fusion protein compris

1995-06-07

1998-11-17

Walsh, Stephen

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

435 691, 4353201, 536 234, 536 241, 514 2, C07K 14435, C07K 14705, C07K 14715, C12N 1512

Patent

active

058378162

ABSTRACT:
Coiled-coil molecular recognition is a primary mode of subunit assembly in a large variety of naturally occurring macromolecular complexes. Here we report the use of coiled-coil mediated solution assembly to form a stable, heteromeric complex of Interleukin-2 receptor ectodomains that binds IL-2 cooperatively and with an affinity approaching the comparable cell surface complex. Cross-linking of homomeric or heteromeric receptor subunits is the common signal transmission mechanism employed by hematopoietin receptors. Individual receptor ectodomains, however, often do not bind ligand with measurable affinity. The claimed invention is useful for coiled-coil mediated assembly of receptor complexes for solution analysis. It also shows that hetero-oligomeric complexes can be formed using a leucine zipper domain.

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